Chitin utilization by the insect-transmitted bacterium Xylella fastidiosa.

Applied and Environmental Microbiology
Nabil KillinyRodrigo P P Almeida

Abstract

Xylella fastidiosa is an insect-borne bacterium that colonizes xylem vessels of a large number of host plants, including several crops of economic importance. Chitin is a polysaccharide present in the cuticle of leafhopper vectors of X. fastidiosa and may serve as a carbon source for this bacterium. Biological assays showed that X. fastidiosa reached larger populations in the presence of chitin. Additionally, chitin induced phenotypic changes in this bacterium, notably increasing adhesiveness. Quantitative PCR assays indicated transcriptional changes in the presence of chitin, and an enzymatic assay demonstrated chitinolytic activity by X. fastidiosa. An ortholog of the chitinase A gene (chiA) was identified in the X. fastidiosa genome. The in silico analysis revealed that the open reading frame of chiA encodes a protein of 351 amino acids with an estimated molecular mass of 40 kDa. chiA is in a locus that consists of genes implicated in polysaccharide degradation. Moreover, this locus was also found in the genomes of closely related bacteria in the genus Xanthomonas, which are plant but not insect associated. X. fastidiosa degraded chitin when grown on a solid chitin-yeast extract-agar medium and grew in liquid medium with chi...Continue Reading

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Citations

Dec 24, 2011·Applied and Environmental Microbiology·Luisa F CruzLeonardo De La Fuente
Nov 22, 2011·Applied and Environmental Microbiology·Nabil KillinyRodrigo P P Almeida
May 18, 2013·Molecular Plant-microbe Interactions : MPMI·N KillinyR P P Almeida
Oct 8, 2013·Biotechnology Advances·Sina Adrangi, Mohammad Ali Faramarzi
Apr 7, 2016·Molecular Plant-microbe Interactions : MPMI·Fabien LabroussaaRodrigo P P Almeida
Feb 18, 2017·Marine Drugs·Alexander J WinklerMarta Berrocal-Lobo
Feb 1, 2017·Microbiology·Fabien LabroussaaRodrigo P P Almeida
Mar 3, 2017·Medical Mycology Journal·Sanae A IshijimaShigeru Abe
Sep 20, 2015·Applied and Environmental Microbiology·Jeannette N RapicavoliM Caroline Roper
Nov 5, 2013·Applied and Environmental Microbiology·Nabil Killiny, Rodrigo P P Almeida
Jul 30, 2020·Insects·Mariana Bossi EstevesJoão Roberto Spotti Lopes
May 23, 2020·Biotechnology Advances·Wei ChenQing Yang

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