PMID: 8987504Sep 1, 1996Paper

Chitinase and beta-1,3-glucanase in the lutoid-body fraction of Hevea latex

Phytochemistry
T SubrotoJ J Beintema

Abstract

The lutoid-body (bottom) fraction of latex from the rubber tree (Hevea brasiliensis) contains a limited number of major proteins. These are, besides the chitin-binding protein hevein, its precursor and the C-terminal fragment of this precursor, proteins with enzymic activities: three hevamine components, which are basic, vacuolar, chitinases with lysozyme activity, and a beta-1,3-glucanase. Lutoid-body fractions from three rubber-tree clones differed in their contents of these enzyme proteins. The hevamine components and glucanase were isolated and several enzymic and structural properties were investigated. These enzymes are basic proteins and cause coagulation of the negatively charged rubber particles. The coagulation occurs in a rather narrow range of ratios of added protein to rubber particles, which indicates that charg neutralization is the determining factor. Differences in coagulation of rubber particles by lutoid-body fractions from various rubber clones can be explained by their content of hevamine and glucanase. Glucanase from the lutoid-body fraction may dissolve callus tissue and this may explain the observation that rubber-tree clones with a high glucanase content in this fraction produce more latex than clones w...Continue Reading

References

Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·I BroekaertN Raikhel
Nov 16, 1994·Biochimica Et Biophysica Acta·U M SoedjanaatmadjaJ J Beintema
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Citations

Aug 10, 2002·Phytochemistry·Dhirayos WititsuwannakulRapepun Wititsuwannakul
Jun 25, 2002·Methods : a Companion to Methods in Enzymology·H Y YeangE Sunderasan
Jan 14, 2004·European Journal of Biochemistry·Bjørnar SynstadVincent G H Eijsink
Jul 17, 2009·Plant Physiology·Daniela WahlerDirk Prüfer
Mar 27, 1998·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A PoschX Baur
Sep 22, 2012·Plant Physiology and Biochemistry : PPB·Qiulan XiangRizhong Zeng
Jun 19, 2016·Biochimie·Karine BerthelotSophie Lecomte
Oct 7, 2016·Plant Physiology and Biochemistry : PPB·Zheng TongXuchu Wang
Feb 9, 2017·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Mohd Afiq Hazlami HabibMohd Nazri Ismail
Sep 15, 2005·Journal of Agricultural and Food Chemistry·Chui-Liang ChiangHsien-Yi Sung

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