Abstract
The lutoid-body (bottom) fraction of latex from the rubber tree (Hevea brasiliensis) contains a limited number of major proteins. These are, besides the chitin-binding protein hevein, its precursor and the C-terminal fragment of this precursor, proteins with enzymic activities: three hevamine components, which are basic, vacuolar, chitinases with lysozyme activity, and a beta-1,3-glucanase. Lutoid-body fractions from three rubber-tree clones differed in their contents of these enzyme proteins. The hevamine components and glucanase were isolated and several enzymic and structural properties were investigated. These enzymes are basic proteins and cause coagulation of the negatively charged rubber particles. The coagulation occurs in a rather narrow range of ratios of added protein to rubber particles, which indicates that charg neutralization is the determining factor. Differences in coagulation of rubber particles by lutoid-body fractions from various rubber clones can be explained by their content of hevamine and glucanase. Glucanase from the lutoid-body fraction may dissolve callus tissue and this may explain the observation that rubber-tree clones with a high glucanase content in this fraction produce more latex than clones w...Continue Reading
References
Aug 15, 1991·European Journal of Biochemistry·P A JekelJ J Beintema
Nov 25, 1990·Nucleic Acids Research·G GheysenC Castresana
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·I BroekaertN Raikhel
Aug 15, 1970·Nature·U K Laemmli
Dec 15, 1994·Structure·A C Terwisscha van ScheltingaB W Dijkstra
Apr 24, 1995·FEBS Letters·U M SoedjanaatmadjaJ J Beintema
Nov 16, 1994·Biochimica Et Biophysica Acta·U M SoedjanaatmadjaJ J Beintema
Apr 1, 1994·The Plant Journal : for Cell and Molecular Biology·L S MelchersH J Linthorst
Aug 1, 1993·The Biochemical Journal·B Henrissat, A Bairoch
May 1, 1960·The Biochemical Journal·B L ARCHER
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·H ShinshiF Meins
Feb 1, 1991·Plant Physiology·M N Martin
Citations
Feb 9, 2011·Plant Cell Reports·Deguan TanJiaming Zhang
Mar 13, 2012·Planta·Manassawe LertpanyasampathaJarunya Narangajavana
Aug 10, 2002·Phytochemistry·Dhirayos WititsuwannakulRapepun Wititsuwannakul
Jun 18, 2003·Phytochemistry·Peter A JekelJaap J Beintema
Jun 25, 2002·Methods : a Companion to Methods in Enzymology·H Y YeangE Sunderasan
Feb 16, 2002·European Journal of Biochemistry·Evert BokmaJaap J Beintema
Jan 14, 2004·European Journal of Biochemistry·Bjørnar SynstadVincent G H Eijsink
Jul 17, 2009·Plant Physiology·Daniela WahlerDirk Prüfer
Apr 26, 2005·Bioscience, Biotechnology, and Biochemistry·Toki TairaMasanobu Ishihara
May 17, 2014·Phytochemistry·Pannawich Sukprasirt, Rapepun Wititsuwannakul
Mar 27, 1998·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A PoschX Baur
Sep 22, 2012·Plant Physiology and Biochemistry : PPB·Qiulan XiangRizhong Zeng
Jul 26, 2006·Plant Biology·E de Castro MiguelM Da Cunha
Apr 26, 2016·Scientific Reports·Dejun LiChengzhi Liang
Nov 16, 2004·Carbohydrate Research·Akamol KlaikherdMongkol Sukwattanasinitt
Jun 19, 2016·Biochimie·Karine BerthelotSophie Lecomte
Oct 7, 2016·Plant Physiology and Biochemistry : PPB·Zheng TongXuchu Wang
Feb 9, 2017·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Mohd Afiq Hazlami HabibMohd Nazri Ismail
May 25, 2001·The Journal of Biological Chemistry·S HuecasR Rodríguez
Jul 26, 2003·Plant & Cell Physiology·Panida Kongsawadworakul, Hervé Chrestin
May 2, 2003·Plant & Cell Physiology·Jin Sun KimHunseung Kang
Jan 9, 2019·BMC Plant Biology·Minjing ShiWei-Min Tian
Mar 17, 2004·The Journal of Biological Chemistry·Siti Arija M ArifHoong-Yeet Yeang
Dec 4, 2019·Phytochemistry·Sunil KumarAshok Kumar Patel
May 15, 2021·Planta·Tomoya TakashimaToki Taira
Sep 15, 2005·Journal of Agricultural and Food Chemistry·Chui-Liang ChiangHsien-Yi Sung