Chitosan activated with divinyl sulfone: a new heterofunctional support for enzyme immobilization. Application in the immobilization of lipase B from Candida antarctica

International Journal of Biological Macromolecules
Bruna B PinheiroLuciana R B Gonçalves

Abstract

A novel heterofunctional support for enzyme immobilization, chitosan-divinyl sulfone, was assessed in this study. The activation of chitosan with DVS was carried out at three different pHs (10.0, 12.5 and 14.0) and a Candida antarctica Lipase B (CALB) was selected as the model enzyme. After immobilization, the biocatalysts were incubated under alkaline conditions in a buffer to facilitate the multipoint covalent attachment, followed by incubation in ethylenediamine (EDA) aiming at blocking the remaining reactive groups. The highest thermal stability was obtained when pH 10.0 was used during support activation. These results were shown to be better than those obtained when using glutaraldehyde as the support-activating reagent. Subsequently, the immobilization pH was investigated (5.0, 7.0 and 10.0) prior to alkaline incubation, with the highest enzyme stability levels found at pH 10.0. Finally, the selected biocatalyst was used in the hydrolysis of ethyl hexanoate and presented an activity of 14,520.37 U/g of immobilized lipase at pH 5.0. These results show that chitosan activated with divinyl sulfone is a very promising support for enzyme immobilization and the proposed protocol is able to successfully improve enzyme stability.

Citations

May 20, 2020·3 Biotech·José E S SouzaJosé C S Dos Santos
Aug 8, 2020·Journal of Oleo Science·Fenghuan ZhaoNanjing Zhong
Aug 21, 2019·International Journal of Molecular Sciences·Rodolpho R C MonteiroRoberto Fernandez-Lafuente
Nov 3, 2020·Preparative Biochemistry & Biotechnology·Bruno Matheus SimõesPatrícia Salomão Garcia
Jul 16, 2020·Biotechnology Advances·Sara Arana-PeñaRoberto Fernandez-Lafuente
Aug 5, 2019·International Journal of Biological Macromolecules·JunQing QianHui Guo
Sep 7, 2019·International Journal of Biological Macromolecules·Nathalia Saraiva RiosLuciana Rocha Barros Gonçalves
Feb 7, 2021·International Journal of Biological Macromolecules·Muhammad BilalLuiz Fernando Romanholo Ferreira
Jan 22, 2021·International Journal of Biological Macromolecules·Shang WangXiao-Wei Yu
Jan 2, 2021·International Journal of Biological Macromolecules·Laura Fonseca FerreiraMarali Vilela Dias
Jan 23, 2021·Biotechnology and Applied Biochemistry·Paula Jéssyca Morais LimaJosé Cleiton Sousa Dos Santos
Mar 6, 2021·International Journal of Biological Macromolecules·F Rafiee, M Rezaee
Apr 18, 2021·International Journal of Biological Macromolecules·Yale Luck NunesJosé C S Dos Santos
Jun 7, 2021·International Journal of Biological Macromolecules·Eduardo Silveira RibeiroPatrícia Silva Diaz
May 31, 2021·Biotechnology and Applied Biochemistry·Michael da Conceição de CastroAlessandra Machado Baron
Jul 17, 2021·Critical Reviews in Biotechnology·Marcin KublickiRyszard Ostaszewski
Aug 11, 2021·International Journal of Biological Macromolecules·Veymar G Tacias-PascacioRoberto Fernandez-Lafuente
Aug 30, 2021·Biotechnology Advances·Rafael C RodriguesRoberto Fernandez-Lafuente

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