Chitosan and its quaternized derivative as effective long dsRNA carriers targeting shrimp virus in Spodoptera frugiperda 9 cells

Journal of Biotechnology
Gatesara TheerawanitchpanVanvimon Saksmerprome

Abstract

RNA interference (RNAi) is a promising strategy to combat shrimp viral pathogens at lab-scale experiments. Development of effective orally delivered agents for double-stranded (ds)RNA is necessary for RNAi application at farm level. Since continuous shrimp cell lines have not been established, we are developing a dsRNA-delivery system in Spodoptera frugiperda (Sf9) cells for studying in vitro RNAi-mediated gene silencing of shrimp virus. Sf9 cells challenged with yellow head virus (YHV) were used for validating nanoparticles as effective dsRNA carriers. Inexpensive and biodegradable polymers, chitosan and its quarternized derivative (QCH4), were formulated with long dsRNA (>100 bp) targeting YHV. Their morphology and physicochemical properties were examined. When treated with chitosan- and QCH4-dsRNA complexes, at least 50% reduction in YHV infection in Sf9 cells relative to the untreated control was evident at 24h post infection with low cytoxicity. Inhibitory effects of chitosan- and QCH4-dsRNA complexes were comparable to that of dsRNA formulated with Cellfectin(®), a commercial lipid-based transfection reagent. The natural and quaternized chitosan prepared in this study can be used for shrimp virus-specific dsRNA delivery i...Continue Reading

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Citations

Apr 19, 2015·International Journal of Pharmaceutics·Bhavani MiryalaKaushal Rege
Apr 17, 2020·Archives of Insect Biochemistry and Physiology·Dhandapani GurusamySubba Reddy Palli
Apr 6, 2021·Frontiers in Cellular and Infection Microbiology·Homa BoroumandHamed Mirzaei
Jul 18, 2021·Journal of Food Science·Josemar Gonçalves de Oliveira FilhoMariana Buranelo Egea

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