Chitosan-based membrane chromatography for protein adsorption and separation

Materials Science & Engineering. C, Materials for Biological Applications
Yezhuo LiuXin Chen

Abstract

A chitosan-based membrane chromatography was set up by using natural chitosan/carboxymethylchitosan (CS/CMCS) blend membrane as the matrix. The dynamic adsorption property for protein (lysozyme as model protein) was detailed discussed with the change in pore size of the membrane, the flow rate and the initial concentration of the feed solution, and the layer of membrane in membrane stack. The best dynamic adsorption capacity of lysozyme on the CS/CMCS membrane chromatography was found to be 15.3mg/mL under the optimal flow conditions. Moreover, the CS/CMCS membrane chromatography exhibited good repeatability and reusability with the desorption efficiency of ~90%. As an application, lysozyme and ovalbumin were successfully separated from their binary mixture through the CS/CMCS membrane chromatography. This implies that such a natural chitosan-based membrane chromatography may have great potential on the bioseparation field in the future.

References

Jul 30, 2003·Journal of Colloid and Interface Science·Hidetoshi MatsumotoAkihiko Tanioka
Aug 27, 2003·Journal of Chromatography. a·Maria-Elena AvramescuMatthias Wessling
Sep 26, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Cristiana Boi

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Citations

Apr 27, 2016·Carbohydrate Polymers·Xiaomin WangChuanfang Yang

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