Chitosan-coated doxorubicin nano-particles drug delivery system inhibits cell growth of liver cancer via p53/PRC1 pathway

Biochemical and Biophysical Research Communications
Bai-Liang YeHua-Jie Cai

Abstract

Nano-particles have been widely used in target-specific drug delivery system and showed advantages in cancers treatment. This study aims to evaluate the effect of chitosan coated doxorubicin nano-particles drug delivery system in liver cancer. The chitosan nano-particles were prepared by using the ionic gelation method. The characterizations of the nano-particles were determined by transmission electron microscopy. The cytotoxicity was detected by MTT assay, and the endocytosis, cell apoptosis and cell cycle were examined by flow cytometry. The protein level was analyzed with western blot. The dual luciferase reporter assay was performed to assess the interaction between p53 and the promoter of PRC1, and chromatin immune-precipitation was used to verify the binding between them. The FA-CS-DOX nano-particles were irregular and spherical particles around 30-40 nm, with uniform size and no adhesion. No significant difference was noted in doxorubicin release rate between CS-DOX and FA-CS-DOX. FA-CS-DOX nano-particles showed stronger cytotoxicity than CS-DOX. FA-CS-DOX nano-particles promoted the apoptosis and arrested cell cycle at G2/M phase, and they up-regulated p53. FA-CS-DOX nano-particles inhibited cell survival through p53/P...Continue Reading

Citations

Dec 29, 2019·International Journal of Molecular Sciences·Małgorzata Zienkiewicz-StrzałkaIwona Komaniecka
Oct 28, 2020·Environmental Science and Pollution Research International·Helton José AlvesMabel Karina Arantes
Jun 25, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Zhe MaZhidong Liu
Jan 2, 2021·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Hualing XiePing Shao
Sep 20, 2020·Advances in Colloid and Interface Science·Abdur RehmanSohail Khan
Aug 10, 2020·Chemico-biological Interactions·Yoke Ying TanDinesh Kumar Chellappan

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