Chitosan elicitation of saponin accumulation in Psammosilene tunicoides hairy roots by modulating antioxidant activity, nitric oxide production and differential gene expression.

Plant Physiology and Biochemistry : PPB
Hanhan QiuZongshen Zhang

Abstract

Elicitation is one of the most effective strategies for enhancing plant bioactive compounds, such as triterpenoid saponins. Chitosan gained worldwide attention for biostimulant activity, but little is known about its roles in the elicitation of triterpenoid saponin in medicinal plants. Here, we explored the regulatory network of chitosan on saponin accumulation in hairy root cultures of Psammosilene tunicoides, a valuable medicinal herb known for its pain-relieving properties endemic to China. Compared with control, the highest total saponin accumulation exhibited a 4.55-fold enhancement in hairy roots elicited by 200 mg L-1 chitosan for nine days. High-performance liquid chromatography (HPLC) revealed the yields of quillaic acid, gypsogenin and gypsogenin-3-O-β-D-glucuronopyranoside were significantly increased after chitosan treatments. Moreover, exogenous chitosan application dramatically triggered the reactive oxygen species (ROS) scavenging enzyme activities and nitric oxide (NO) content in hairy roots. Comparative transcriptome analysis from chitosan-treated (1 and 9 d) or control groups revealed that differentially expressed genes (DEGs) were greatly enriched in plant-pathogen interaction and metabolic processes. The tra...Continue Reading

References

Dec 18, 2004·Nitric Oxide : Biology and Chemistry·Jian Wen Wang, Jian Yong Wu
Apr 12, 2007·Biotechnology Letters·Waraporn PutalunYukihiro Shoyama
Jan 18, 2011·Journal of Plant Research·Giovanni PoveroPierdomenico Perata
Apr 3, 2013·Carbohydrate Polymers·Zengxin MaXianghong Meng
May 21, 2013·Plant Science : an International Journal of Experimental Plant Biology·Lee A Hadwiger
Jan 7, 2014·Plant Science : an International Journal of Experimental Plant Biology·Philippe G ChatelainMarta W Vasconcelos
Feb 11, 2015·Journal of Environmental Sciences (China)·Liang LiGang Pan
Sep 10, 2015·Plant Physiology and Biochemistry : PPB·Zhongyang XuWanquan Ji
Jan 27, 2018·International Journal of Molecular Sciences·Tao WangZhong-Ren Guo
Aug 21, 2018·International Journal of Biological Macromolecules·Jiawei ZhouWei Gao
Oct 19, 2019·Plant Science : an International Journal of Experimental Plant Biology·Qingguo SunXuesen Chen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Basal Ganglia

Basal Ganglia are a group of subcortical nuclei in the brain associated with control of voluntary motor movements, procedural and habit learning, emotion, and cognition. Here is the latest research.

Related Papers

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica
Jingbin LiZongshen Zhang
Research in Immunology
J B Campbell, Y A Peerbaye
Dansk tidsskrift for farmaci
M von Schantz
© 2021 Meta ULC. All rights reserved