Chitosan films with improved tensile strength and toughness from N-acetyl-cysteine mediated disulfide bonds

Carbohydrate Polymers
Kevin Barrett MilesHoward W T Matthew

Abstract

To improve the mechanical properties of chitosan (Ct) materials without the use of cytotoxic crosslinkers, disulfide cross-linkable Ct was synthesized by grafting N-acetyl-cysteine (NAC) to Ct using carbodiimide chemistry. Cast films of NAC-Ct conjugates were prepared with degrees of substitution (DS) of 0%, 6%, 15%, and 20%, and the disulfide bond formation was induced by increasing the reaction media pH to 11. The tensile strength, breaking strain, elastic moduli and toughness of disulfide cross-linked polymers were analyzed by monotonic tensile testing of hydrated NAC-Ct films. Crystallinity was determined via XRD. Results demonstrated that NAC incorporation and crosslinking in chitosan produced tougher polymer films with 4-fold higher tensile strength (10 MPa) and 6-fold greater elongation (365%), but reduced crystallinity, compared to unmodified chitosan. The resilience of NAC-Ct films was evaluated by cyclic testing, and results demonstrate that increasing NAC content produced a more resilient material that dissipated less energy when deformed. These improved mechanical properties broaden chitosan's applicability towards the construction of mechanically robust implantable scaffolds for tissue regeneration.

References

May 1, 1988·Biomaterials·R MuzzarelliV Vasi
Jan 1, 1973·Biopolymers·W A HiltnerA G Walton
Sep 1, 1996·Biomaterials·K S AnsethL Brannon-Peppas
Feb 18, 1999·Biomaterials·H Onishi, Y Machida
Jun 26, 1999·Biomaterials·S V Madihally, H W Matthew
Jan 5, 2002·Journal of Biomedical Materials Research·Pamela J VandeVordPaul H Wooley
Mar 26, 2002·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·John GoslineKen Savage
May 10, 2002·Journal of Biomedical Materials Research·Julie E GoughSandra Downes
Nov 12, 2002·Biomacromolecules·Xiao Zheng ShuGlenn D Prestwich
Aug 9, 2003·Journal of Pharmaceutical and Biomedical Analysis·M LavertuAjay Gupta
Jan 3, 2006·Journal of Materials Science. Materials in Medicine·Ying WanDijiang Wen
Oct 6, 2006·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·M PrabaharanJ F Mano
Mar 27, 2007·Bioresource Technology·Entsar S AbdouMaher Z Elsabee
Aug 8, 2007·International Journal of Pharmaceutics·Thierry SchmitzAndreas Bernkop-Schnürch
Jun 6, 2009·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Z M WuD M Yu
Jul 22, 2010·Journal of Biomedical Materials Research. Part a·Le-Ping YanRui L Reis

❮ Previous
Next ❯

Citations

Dec 14, 2017·Scientific Reports·Fabíola CostaM Cristina L Martins
Dec 11, 2020·Journal of the Mechanical Behavior of Biomedical Materials·Elif IlhanOguzhan Gunduz
Apr 18, 2021·International Journal of Biological Macromolecules·Yale Luck NunesJosé C S Dos Santos
Jun 23, 2020·Biomacromolecules·Christoph FedererAndreas Bernkop-Schnürch

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

© 2022 Meta ULC. All rights reserved