Chitosan microparticles based polyelectrolyte complex scaffolds for bone tissue engineering in vitro and effect of calcium phosphate

Carbohydrate Polymers
Janitha M UnagollaAmbalangodage C Jayasuriya

Abstract

Chitosan microparticles were mixed with chitosan and carboxymethyl cellulose solution to achieve a good binding between the microparticles. Three different compositions of scaffolds were made by varying the calcium phosphate (CaP) amount: 0%, 10%, and 20%. Potassium chloride was used as salt, to make pores inside the scaffolds after leaching out when immersed in phosphate buffer saline (PBS). Compressive strength and compressive modulus of both non-porous (before leaching out), and porous (after leaching out) scaffolds were measured according to the ASTM standards. The highest compressive strength of 27 MPa was reported on 10% CaP scaffolds while 20% CaP scaffolds showed the lowest. The increasing CaP content reduces the compressive strength of the scaffolds. The highest wet state compressive strength was reported on 0% CaP scaffolds with 0.36 MPs and 0.40 MPa at day 1 and day 3 respectively. In vitro cell culture studies showed good cell adhesion and cell proliferation on 10% CaP scaffolds.

Citations

Nov 14, 2020·International Journal of Biological Macromolecules·Mozhgan Keshavarz, Parvin Alizadeh
Feb 23, 2020·International Journal of Biological Macromolecules·Bhisham Narayan SinghPradeep Srivastava
Apr 20, 2019·International Journal of Biological Macromolecules·Bhisham Narayan SinghPradeep Srivastava
Jul 16, 2021·ACS Biomaterials Science & Engineering·Andreja Dobaj ŠtiglicTamilselvan Mohan
Aug 5, 2021·Journal of Biomaterials Science. Polymer Edition·P R SivashankariM Prabaharan
Dec 27, 2018·International Journal of Biological Macromolecules·Yau-Hung ChenTrong-Ming Don

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