Chitosan/double-stranded RNA nanoparticle-mediated RNA interference to silence chitin synthase genes through larval feeding in the African malaria mosquito (Anopheles gambiae)

Insect Molecular Biology
X ZhangK Y Zhu

Abstract

The purpose of this study was to examine whether the expression of two chitin synthase genes, AgCHS1 and AgCHS2, can be repressed by chitosan/AgCHS dsRNA-based nanoparticles through larval feeding in Anopheles gambiae. The AgCHS1 transcript level and chitin content were reduced by 62.8 and 33.8%, respectively, in the larvae fed on chitosan/AgCHS1 dsRNA nanoparticles compared with those of the control larvae fed on chitosan/GFP dsRNA nanoparticles. Our study suggested for the first time that RNA interference (RNAi) in mosquito larvae is systemic, and demonstrated that the larvae fed on the nanoparticles assembled from AgCHS1 and AgCHS2 dsRNA increased larval susceptibilities to diflubenzuron, and calcofluor white (CF) or dithiothreitol, respectively. These results suggest great potential for using such a nanoparticle-based RNAi technology for high-throughput screening of gene functions and for developing novel strategies for pest management.

References

Mar 4, 2000·Insect Biochemistry and Molecular Biology·P Wang, R R Granados
Oct 25, 2000·Insect Biochemistry and Molecular Biology·R L Tellam, C Eisemann
May 29, 2001·Archives of Insect Biochemistry and Physiology·W R Terra
May 29, 2001·Archives of Insect Biochemistry and Physiology·L ShaoM Jacobs-Lorena
Feb 9, 2002·Science·William M WinstonCraig P Hunter
Sep 15, 2004·Development Genes and Evolution·Yoshinori Tomoyasu, Robin E Denell
Sep 17, 2004·Nature·Craig C Mello, Darryl Conte
Aug 3, 2005·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Hans Merzendorfer
Oct 6, 2005·BMC Biotechnology·Ying Dong, Markus Friedrich
Mar 15, 2006·The Journal of Biological Chemistry·Johanna UlvilaMika Rämet
Jul 11, 2006·Molecular Therapy : the Journal of the American Society of Gene Therapy·Kenneth A HowardJørgen Kjems
Jul 25, 2006·Nature Cell Biology·Maria-Carla SalehRaul Andino
Sep 9, 2006·Journal of Controlled Release : Official Journal of the Controlled Release Society·Haliza Katas, H Oya Alpar
Dec 13, 2006·Biotechnology and Bioengineering·Derek W Bartlett, Mark E Davis
Jun 15, 2007·Proceedings of the National Academy of Sciences of the United States of America·William M WinstonCraig P Hunter
Jun 26, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Dana J GaryYou-Yeon Won
Nov 6, 2007·Nature Biotechnology·James A BaumJames Roberts
Nov 9, 2007·Nature Biotechnology·Karl H J Gordon, Peter M Waterhouse
May 10, 2008·Journal of Microencapsulation·Crispin R Dass, Peter F M Choong
Jul 16, 2008·Insect Biochemistry and Molecular Biology·Xuguo ZhouMichael E Scharf
Jul 30, 2008·Development Genes and Evolution·Sherry C MillerYoshinori Tomoyasu
Aug 23, 2008·Insect Biochemistry and Molecular Biology·Yasuyuki ArakaneRichard W Beeman
Nov 20, 2008·Insect Molecular Biology·D P WalsheL R Haines
Dec 4, 2008·Insect Biochemistry and Molecular Biology·Francis Morais Franco Nunes, Zilá Luz Paulino Simões
Dec 11, 2008·Annual Review of Entomology·Dwayne HegedusUmut Toprak
Apr 30, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Shan GaoJørgen Kjems
Oct 10, 2009·Insect Biochemistry and Molecular Biology·Steven WhyardSylvia Wong

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Citations

May 9, 2012·Parasitology Research·Tapan K BarikArunava Gowswami
Aug 2, 2013·Applied Biochemistry and Biotechnology·Rajan KatochLarry L Murdock
Mar 31, 2012·Parasitology·Murray E SelkirkCollette Britton
Aug 9, 2013·Parasites & Vectors·Santhosh PuthiyakunnonXiaoguang Chen
May 23, 2013·PLoS Neglected Tropical Diseases·Keshava MysoreMolly Duman-Scheel
Nov 10, 2012·International Journal of Biological Sciences·Raman BansalAndy P Michel
Aug 21, 2013·Transgenic Research·Jianjun Mao, Fanrong Zeng
Jan 23, 2016·International Journal of Biological Macromolecules·D Ramesh KumarS Ignacimuthu
Feb 9, 2012·Journal of Medical Entomology·Giancarlo Lopez-MartinezDavid L Denlinger
Nov 10, 2015·Journal of Insect Physiology·Zhi Xian LimNeena Mitter
Jun 12, 2012·Insect Biochemistry and Molecular Biology·Xin ZhangKun Yan Zhu
Apr 28, 2012·Current Opinion in Microbiology·Christian Mitri, Kenneth D Vernick
Mar 21, 2012·Journal of Invertebrate Pathology·Lyric C BartholomayD L Harris
Feb 14, 2012·Journal of Insect Physiology·Wen-Juan WuChuan-Xi Zhang
Jun 28, 2011·European Journal of Cell Biology·Hans Merzendorfer
Mar 10, 2016·Experimental Parasitology·Pongsopee AttasartWilai Tienrungroj Noonpakdee
Sep 1, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Kathleen A RossBalaji Narasimhan
Mar 18, 2016·Annual Review of Entomology·Kun Yan ZhuSubbaratnam Muthukrishnan
Jul 22, 2014·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Keshava MysoreMolly Duman-Scheel
Aug 29, 2013·Insect Molecular Biology·J Figueira-MansurM F Moreira
Mar 2, 2013·Journal of Biophotonics·Stephen R FosterVladimir P Zharov
Apr 30, 2015·Molecular Genetics and Genomics : MGG·Guo-Dong ZhaoZheng-Guo Wei

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