Chlamydia protein Pgp3 studied at high resolution in a new crystal form

IUCrJ
Sahir KhurshidNaomi E Chayen

Abstract

The protein Pgp3 is implicated in the sexually transmitted disease chlamydia and comprises an extended complex arrangement of a C-terminal domain (CTD) and an N-terminal domain (NTD) linked by a triple-helix coiled coil (THCC). Here, the X-ray crystal structure of Pgp3 from an LGV1 strain is reported at the highest X-ray diffraction resolution obtained to date for the full protein. The protein was crystallized using a high concentration of potassium bromide, which resulted in a new crystal form with relatively low solvent content that diffracted to a resolution of 1.98 Å. The three-dimensional structure of this new crystal form is described and compared with those of other crystal forms, and the potassium bromide binding sites and the relevance to chlamydia isolates from around the globe are described. The crystal packing is apparently driven by the CTDs. Since the threefold axes of the THCC and NTD are not collinear with the threefold axis of a CTD, this naturally leads to disorder in the THCC and the portion of the NTD that does not directly interact with the CTD via crystal packing. The key avenue to resolving these oddities in the crystal structure analysis was a complete new analysis in space group P1 and determining the s...Continue Reading

References

Jun 1, 1997·Microbiology·N S ThomasI N Clarke
May 4, 2000·Protein Science : a Publication of the Protein Society·E Saridakis, N E Chayen
Aug 29, 2002·Acta Crystallographica. Section D, Biological Crystallography·Santosh Panjikar, Paul A Tucker
Aug 1, 2007·Journal of Applied Crystallography·Airlie J McCoyRandy J Read
Jan 9, 2010·Acta Crystallographica. Section D, Biological Crystallography·Vincent B ChenDavid C Richardson
Apr 13, 2010·Acta Crystallographica. Section D, Biological Crystallography·P EmsleyK Cowtan
May 28, 2010·The Journal of Infectious Diseases·Catherine L HaggertyRoberta B Ness
Sep 21, 2010·Journal of Bacteriology·Ding ChenGuangming Zhong
Apr 5, 2011·Acta Crystallographica. Section D, Biological Crystallography·Martyn D WinnKeith S Wilson
Apr 5, 2011·Acta Crystallographica. Section D, Biological Crystallography·Garib N MurshudovAlexei A Vagin
Apr 5, 2011·Acta Crystallographica. Section D, Biological Crystallography·S McNicholasM E M Noble
Oct 12, 2011·The Journal of Experimental Medicine·Laszlo KariHarlan D Caldwell
Apr 17, 2012·Acta Crystallographica. Section D, Biological Crystallography·Pavel V AfoninePaul D Adams
May 25, 2013·The Journal of Biological Chemistry·Ahmad GalaleldeenP John Hart
Nov 12, 2013·Pathogens and Disease·Lihua SongHarlan D Caldwell
Sep 10, 2014·Acta Crystallographica. Section D, Biological Crystallography·Andrey A Lebedev, Michail N Isupov

❮ Previous
Next ❯

Citations

Mar 12, 2020·Microorganisms·Charlotte A JonesColette E O'Neill

❮ Previous
Next ❯

Methods Mentioned

BETA
X-ray
PCR

Software Mentioned

MolProbity
SCALA
MOSFLM
Phaser MR
CCP
Phaser
REFMAC
Coot

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Acta Crystallographica. Section D, Biological Crystallography
Jirí BryndaJuraj Sedlácek
Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Sheng Chia ChenYeh Chen
Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Xingyou YeXiaopeng Hu
Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Hirofumi KomoriYoshiki Higuchi
Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
M Obayed UllahBostjan Kobe
© 2022 Meta ULC. All rights reserved