Abstract
Chlamydia trachomatis is a nucleotide parasite, being entirely dependent on its host eukaryotic cell for a supply of ATP, GTP, and UTP. Chlamydiae are not, however, auxotrophic for CTP, as they are able both to transport CTP from the host and synthesize CTP de novo via a chlamydial CTP synthetase. This study addresses the developmental regulation of CTP synthetase over the course of the C. trachomatis life cycle. Given the distinct life stages of C. trachomatis, analysis of temporal changes in gene expression and regulation of protein activity is the key to unravelling the mechanism of pathogenesis of this bacterium. The results of immunodetection analysis indicate that CTP synthetase is present in C. trachomatis elementary bodies and reticulate bodies and that it is widespread in other chlamydial strains. Reverse transcriptase-polymerase chain reaction (RT-PCR) and metabolic labelling experiments show that CTP synthetase is transcribed and translated primarily during the mid- and late stages of the chlamydial growth cycle. In addition, C. trachomatis CTP synthetase was transcribed with the CTP utilizing enzyme CMP-2-keto-3-deoxy-octanoic acid synthetase (CMP-KDO synthetase) as part of a polycistronic mRNA. The co-expression of...Continue Reading
Citations
Oct 24, 2006·Journal of Bacteriology·P Scott Hefty, Richard S Stephens
Jan 1, 1997·Annual Review of Microbiology·A P Sinai, K A Joiner
Mar 6, 2013·PLoS Genetics·Ghows Azzam, Ji-Long Liu
Jun 7, 2014·Protein Engineering, Design & Selection : PEDS·Mengzhu ZhuJingjing Xie
Sep 21, 2011·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Kangni ChenJi-Long Liu
Dec 17, 2009·Current Opinion in Microbiology·Hector Alex Saka, Raphael H Valdivia
Apr 29, 2008·Progress in Lipid Research·Yu-Fang Chang, George M Carman
Apr 30, 1999·FEMS Microbiology Letters·A G WalshJ S Lam
Jul 1, 2016·Annual Review of Cell and Developmental Biology·Ji-Long Liu
Sep 14, 2000·The Journal of Biological Chemistry·C RoshickG McClarty
Jun 10, 2005·Oral Microbiology and Immunology·C E ShelburneD E Lopatin
Jul 20, 1999·Molecular Microbiology·E R Iliffe-Lee, G McClarty
Nov 5, 2019·Frontiers in Microbiology·Manli YangThomas Dandekar
Apr 7, 2018·Frontiers in Cellular and Infection Microbiology·Heidy Y Narvaez-OrtizBarbara H Zimmermann
Apr 1, 1997·Infection and Immunity·J L WylieG McClarty
Jul 21, 2012·Genome Research·Christina A CuomoEmily R Troemel