Chlamydia trachomatis infection of human endometrial stromal cells induces defective decidualisation and chemokine release.

Scientific Reports
Sevi GiakoumelouAndrew W Horne

Abstract

Miscarriage affects ~20% of pregnancies and maternal infections account for ~15% of early miscarriages. Chlamydia trachomatis (Ct) has been associated with miscarriage but the underlying mechanisms are unknown. Successful implantation requires endometrial stromal cell (ESC) decidualisation. Maintenance of pregnancy requires angiogenesis, establishment of the correct cellular milieu and trophoblast invasion, all of which involve the action of chemokines. Our objective was to determine whether Ct infection impacts upon ESC decidualisation and chemokine secretion. Human primary ESC were decidualised in-vitro, infected with Ct serovar E, and changes in expression of genes of interest were measured using RT-PCR, proteomic array and ELISA. We demonstrate for the first time that Ct can infect and proliferate in ESC. Expression of the decidualisation marker prolactin was decreased in Ct-infected ESC at both mRNA and protein levels. Ct infection altered the chemokine profile of decidualised ESC as shown by proteomic array. Chemokines CXCL12 and CXCL16, important for trophoblast invasion, were analysed further and expression was reduced in infected decidualised cells at mRNA and protein levels. Our data indicate that Ct infection of ESC ...Continue Reading

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Citations

Oct 2, 2019·BJOG : an International Journal of Obstetrics and Gynaecology·M Al-MemarD A MacIntyre
Jun 3, 2020·American Journal of Reproductive Immunology : AJRI·Deng AoMing-Qing Li
Jan 24, 2020·American Journal of Reproductive Immunology : AJRI·Jia-Wei ShiMing-Qing Li

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BETA
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MasterPlex® QT
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