Chlamydial development is blocked in host cells transfected with Chlamydophila caviae incA

BMC Microbiology
Damir T AlzhanovD D Rockey

Abstract

Chlamydiae produce a set of proteins, termed Inc proteins, that are localized to the inclusion membrane and exposed to the host cell cytosol. Little information exists regarding the interaction of Inc proteins with the eukaryotic cell. To examine these interactions, Vaccinia virus vectors and mammalian plasmid-based systems were used to express inc genes in mammalian cells. Cells transfected with plasmids expressing Chlamydophila caviae incA were not productively infected by C. caviae. Expression of C. caviae incA also reduced inclusion formation by Chlamydia trachomatis, but not to the degree seen for C. caviae. Chlamydia trachomatis incA did not block development of either C. trachomatis or C. caviae. Deletion mutagenesis was used to demonstrate that plasmids encoding either the amino or carboxy-terminal regions of the protein, as well as the changing of a single amino acid within IncA (serine 17) could not block C. caviae infection. Immunoblot analysis of truncated IncA in a Vaccinia virus system provided evidence that serine 17 of C. caviae IncA is a target for phosphorylation. These experiments provide insight into the interaction of Inc proteins with the host cell and introduce a model system where these interactions can ...Continue Reading

References

Jul 1, 1997·Trends in Microbiology·T HackstadtR A Heinzen
Jul 15, 1998·Molecular Microbiology·J P BannantineT Hackstadt
Feb 24, 2001·Cellular Microbiology·T HackstadtE R Fischer
Feb 24, 2001·Cellular Microbiology·S Duclos, M Desjardins

❮ Previous
Next ❯

Citations

May 2, 2008·Medical Microbiology and Immunology·Nicole MüllerUwe Gross
Oct 3, 2007·Infection and Immunity·Claudio CortesBenjamin Wizel
Jan 7, 2009·BMC Microbiology·Damir T AlzhanovDaniel D Rockey
Mar 29, 2012·Comparative and Functional Genomics·Erika I LutterTed Hackstadt
Jul 27, 2005·FEMS Microbiology Reviews·Yasser M Abdelrahman, Robert J Belland
Nov 26, 2013·Cell Communication and Signaling : CCS·Adrian Mehlitz, Thomas Rudel
Oct 7, 2009·Expert Review of Vaccines·Daniel D RockeyGuangming Zhong
Apr 22, 2008·Biomedit︠s︡inskai︠a︡ khimii︠a︡·E S KostriukovaV M Govorun
Nov 8, 2019·PloS One·Kimberly FilcekBarbara S Sixt
Oct 18, 2014·The Journal of Biological Chemistry·Erik RonzoneFabienne Paumet

❮ Previous
Next ❯

Methods Mentioned

BETA
fluorescence microscopy
transfection

Software Mentioned

Canvas
Adobe
GraphPad
Photoshop
Instat
Deneba

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.