Chloral hydrate induced spindle aberrations, metaphase I arrest and aneuploidy in mouse oocytes

Mutagenesis
Ursula Eichenlaub-Ritter, Ilse Betzendahl

Abstract

In view of tissue- and sex-specific differences in chemically-induced aneuploidy, we analysed the effects of chloral hydrate (CH) on cell-cycle progression, spindle formation and aneuploidy in in vitro maturing mouse oocytes with cytogenetic and immunofluorescent methods. CH blocks oocyte maturation irreversibly and concentration dependently. During culture in 125 micrograms/ml CH, germinal vesicle breakdown is delayed, and most oocytes become arrested in meiosis I with bivalent chromosomes. Their spindles are asymmetric or attain fusiform poles. Oocytes which progress to metaphase II also possess astral instead of barrel-shaped, anastral spindles as characteristic for the controls. Resolution of chiasmata without polar body extrusion during exposure to 50 and 125 micrograms/ml CH results in significant rises in 'diploid' metaphase II oocytes. Hyperploidy rates do not increase significantly at any concentration of CH, but hypoploidy levels are elevated at 125 micrograms/ml CH. CH induces lagging of chromosomes during telophase I, inhibits spindle elongation in anaphase B and causes chromosome displacement from the spindle equator in metaphase I and II. Oocytes also become irreversibly arrested in maturation when exposed to CH p...Continue Reading

Citations

Dec 13, 2000·Mutation Research·A Russo
Jan 1, 1999·Maturitas·U Eichenlaub-Ritter
Apr 6, 2006·Reproductive Biology and Endocrinology : RB&E·Hang YinRoger G Gosden
Sep 30, 2005·Cytogenetic and Genome Research·J B Mailhes, F Marchetti
Sep 30, 2005·Cytogenetic and Genome Research·N Steuerwald
Feb 4, 2006·Drug Safety : an International Journal of Medical Toxicology and Drug Experience·Tmirah HaselkornGary D Friedman
Nov 7, 2002·Reproductive Biomedicine Online·Ursula Eichenlaub-RitterHans-Rudolf Tinneberg
Dec 11, 2007·Mutation Research·Fengyun SunUrsula Eichenlaub-Ritter
Dec 28, 2007·Mutation Research·Ying ShenUrsula Eichenlaub-Ritter
Dec 22, 2007·Mutation Research·Ursula Eichenlaub-RitterJim Parry
Jan 1, 1996·Environmental and Molecular Mutagenesis·U Eichenlaub-Ritter
Jan 1, 1996·Environmental and Molecular Mutagenesis·A J WyrobekF Marchetti
Jan 19, 2007·Biology of Reproduction·Ursula Eichenlaub-RitterRalph Sorensen

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