Chlorhexidine-loaded hydroxyapatite microspheres as an antimicrobial delivery system and its effect on in vivo osteo-conductive properties

Journal of Materials Science. Materials in Medicine
Carlos Alberto Soriano-SouzaAlexandre M Rossi

Abstract

Hydroxyapatite (HA) has been investigated as a delivery system for antimicrobial and antibacterial agents to simultaneously stimulate bone regeneration and prevent infection. Despite evidence supporting the bactericidal efficiency of these HA carriers, few studies have focused on the effect of this association on bone regeneration. In this work, we evaluated the physico-chemical properties of hydroxyapatite microspheres loaded with chlorhexidine (CHX) at two different concentrations, 0.9 and 9.1 μgCHX/cm2 HA, and characterized their effects on in vitro osteoblast viability and bone regeneration. Ultraviolet-visible spectroscopy, scanning and transmission electron microscopy associated with energy-dispersive X-ray spectroscopy and electron energy loss spectroscopy were used to characterize the association of CHX and HA nanoparticles. The high CHX loading dose induced formation of organic CHX plate-like aggregates on the HA surface, whereas a Langmuir film was formed at the low CHX surface concentration. Quantitative evaluation of murine osteoblast viability parameters, including adhesion, mitochondrial activity and membrane integrity of cells exposed to HA/CHX extracts, revealed a cytotoxic effect for both loading concentrations...Continue Reading

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Citations

Apr 5, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Rahmatullah CholasAntonio Licciulli
Nov 15, 2017·Biomedical Materials·Antonio Márcio ScatoliniEliana Cristina da Silva Rigo
Sep 10, 2019·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Carlos Soriano-SouzaMônica Calasans-Maia
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Nov 19, 2020·International Journal of Nanomedicine·Suelen Cristina SartorettoMonica Diuana Calasans-Maia
Jan 15, 2021·International Journal of Environmental Research and Public Health·Jhonathan R B NascimentoJosé A Calasans-Maia
May 16, 2020·Materials Science & Engineering. C, Materials for Biological Applications·Suelen C SartorettoWillian F Zambuzzi
Jul 22, 2019·Open Life Sciences·In-Seok SongJun-Beom Park

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