Chloride channels in the small intestinal cell line IEC-18

Journal of Cellular Physiology
Srisaila BasavappaUma Sundaram

Abstract

Small intestinal crypt cells play a critical role in modulating Cl- secretion during digestion. The types of Cl- channels mediating Cl- secretion in the small intestine was investigated using the intestinal epithelial cell line, IEC-18, which was derived from rat small intestine crypt cells. In initial radioisotope efflux studies, exposure to forskolin, ionomycin or a decrease in extracellular osmolarity significantly increased 36Cl efflux as compared to control cells. Whole cell patch clamp techniques were subsequently used to examine in more detail the swelling-, Ca2+-, and cAMP-activated Cl- conductance. Decreasing the extracellular osmolarity from 290 to 200 mOsm activated a large outwardly rectifying Cl- current that was voltage-independent and had an anion selectivity of I- > Cl-. Increasing cytosolic Ca2+ by ionomycin activated whole cell Cl- currents, which were also outwardly rectifying but were voltage-dependent. The increase in intracellular Ca2+ levels with ionomycin was confirmed with fura-2 loaded IEC-18 cells. A third type of whole cell Cl- current was observed after increases in intracellular cAMP induced by forskolin. These cAMP-activated Cl- currents have properties consistent with cystic fibrosis transmembran...Continue Reading

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Citations

Oct 16, 2007·JAAPA : Official Journal of the American Academy of Physician Assistants
Nov 20, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Xing-He WengAndrea Quaroni
Jun 16, 2012·American Journal of Physiology. Gastrointestinal and Liver Physiology·Yan LiJingxin Li

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