Chloride channels regulate HIT cell volume but cannot fully account for swelling-induced insulin secretion

Diabetes
T A KinardL S Satin

Abstract

Insulin-secreting pancreatic islet beta-cells possess anion-permeable Cl- channels (I(Cl,islet)) that are swelling-activated, but the role of these channels in the cells is unclear. The Cl- channel blockers 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS) and niflumic acid were evaluated for their ability to inhibit I(Cl,islet) in clonal beta-cells (HIT cells). Both drugs blocked the channel, but the blockade due to niflumic acid was less voltage-dependent than the blockade due to DIDS. HIT cell volume initially increased in hypotonic solution and was followed by a regulatory volume decrease (RVD). The addition of niflumic acid and, to a lesser extent, DIDS to the hypotonic solution potentiated swelling and blocked the RVD. In isotonic solution, niflumic acid produced swelling, suggesting that islet Cl- channels are activated under basal conditions. The channel blockers glyburide, gadolinium, or tetraethylammonium-Cl did not alter hypotonic-induced swelling or volume regulation. The Na/K/2Cl transport blocker furosemide produced cell shrinkage in isotonic solution and blocked cell swelling normally induced by hypotonic solution. Perifused HIT cells secreted insulin when challenged with hypotonic solutions. However, this ...Continue Reading

References

Oct 1, 1992·The Journal of Physiology·M Kubo, Y Okada
Apr 1, 1992·The Journal of Physiology·P Doroshenko, E Neher
Dec 11, 1991·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·S E KahnD Porte
Oct 23, 1990·European Journal of Pharmacology·P E Sandström
Jan 31, 1991·Biochimica Et Biophysica Acta·K G EngströmJ Sehlin
Apr 13, 1990·Biochimica Et Biophysica Acta·P E Sandström, J Sehlin
Jan 1, 1990·Annual Review of Physiology·W Almers
Apr 1, 1988·Pflügers Archiv : European journal of physiology·L S Satin, D L Cook
Jun 1, 1989·The American Journal of Physiology·R T WorrellR A Frizzell
Jan 1, 1989·Progress in Biophysics and Molecular Biology·F M Ashcroft, P Rorsman
Apr 1, 1989·Physiological Reviews·E K Hoffmann, L O Simonsen
Jan 1, 1985·The American Journal of Physiology·G T EddlestoneP M Beigelman
Jun 1, 1988·The Journal of Physiology·P LindströmJ Sehlin
Nov 1, 1988·The American Journal of Physiology·P E Sandström, J Sehlin
Dec 1, 1986·Acta Physiologica Scandinavica·P LindströmJ Sehlin
Jun 12, 1986·Biochimica Et Biophysica Acta·E K Hoffmann
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Jul 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·R F SanterreC P Wilson
Apr 29, 1983·Biochemical and Biophysical Research Communications·J C Henquin, H P Meissner
Nov 1, 1994·The Journal of Membrane Biology·S FatheraziC M Belton
Jun 1, 1993·Current Opinion in Neurobiology·T J Jentsch
Jan 1, 1996·Pflügers Archiv : European journal of physiology·L BestP D Brown
Mar 1, 1996·The Journal of Biological Chemistry·L MissiaenR Casteels
Mar 1, 1996·The American Journal of Physiology·K StrangeP S Jackson
Mar 1, 1996·The Journal of Membrane Biology·M Coca-PradosM M Civan
Dec 1, 1995·Pflügers Archiv : European journal of physiology·T MoserE Neher

❮ Previous
Next ❯

Citations

Dec 18, 2001·Diabetes/metabolism Research and Reviews·B NazlielB Koçer
Aug 27, 2005·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Zuzana BacováVladimir Strbák
Aug 18, 2006·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Martin JakabMarkus Ritter
Mar 11, 2004·The European Journal of Neuroscience·Karina TuzHerminia Pasantes-Morales
Feb 27, 2008·Journal of Cellular Physiology·Rodrigo FrancoLenin D Ochoa de la Paz
Oct 8, 2009·Cell Metabolism·Ludmila V DeriyDeborah J Nelson
Sep 7, 2004·The Journal of Biological Chemistry·Charles F SimardPaul Isenring
Sep 25, 2015·The Journal of Endocrinology·Saeed AlshahraniMauricio Di Fulvio
Jan 17, 2019·American Journal of Physiology. Cell Physiology·Toshiaki SawataniTomohisa Ishikawa
May 19, 2018·Nature Communications·Till StuhlmannThomas J Jentsch
Dec 30, 2014·American Journal of Physiology. Endocrinology and Metabolism·Meirigeng QiIsmail H Al-Abdullah
Apr 6, 2002·American Journal of Physiology. Endocrinology and Metabolism·Susanne G StraubGeoffrey W G Sharp
Nov 16, 2019·Scientific Reports·Vijayalakshmi DeivasikamaniPiruthivi Sukumar
Jul 11, 2006·American Journal of Physiology. Cell Physiology·Miki TakiiKoichi Nakayama
May 13, 2017·Scientific Reports·Shams KursanMauricio Di Fulvio

❮ Previous
Next ❯

Related Concepts

Related Feeds

American Diabetes Association Journals

Discover the latest diabetes research published by the journals from the American Diabetes Association.