Abstract
Cultured astrocytoma cells were voltage clamped with pipettes where [Ca2+] in the pipette was buffered to 10(-7) M with 10 mM 1,2-bis(O-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA) and membrane currents recorded. In isotonic solution predominantly K+ currents were evoked by depolarizing or hyperpolarizing commands and 4,4'-diisothiocyanatostilbene-2,2'-disulphonic acid (DIDS; 1 mM) had little effect on inward and outward currents evoked by voltage steps to negative and positive potentials. If the osmolarity of the bathing solution was reduced from 280 to 200 mosmol l-1, a large current developed, which rectified outwardly and reversed close to the equilibrium potential for chloride ions, ECl. Upon stepping to potentials positive to about +30 mV in hypotonic solution, this outward current inactivated over 2-3 s; this decline was greater with N-methyl-D-glucamine chloride (NMDG-Cl) in the pipette than when KCl or sodium glutamate was present. Holding at various positive potentials inactivated the current, with half-inactivation occurring around +50 mV. The current reactivated over 2-3 s at potentials negative to about +30 mV. The current evoked by hypotonic solution was inhibited by various putative chloride channel blo...Continue Reading
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