Chloride ion channels are associated with adherence of lymphatic endothelial cells

Microvascular Research
D K MartinT J Campbell

Abstract

We have examined the role of chloride ion channels as part of the control mechanism for adherence and growth of lymphatic endothelial cells. The chloride channel inhibitor N-phenylanthranilic acid (0.1 mM) inhibited the initial adherence of previously nonadherent cells in the period up to 3 hr following seeding onto gelatin-coated culture dishes. The potassium channel inhibitor 4-aminopyridine (1 mM) had no effect on the rate of cell adherence. N-Phenylanthranilic acid had no effect when added to confluent monolayers of cells that had been growing for 3 days. This, and the observation that N-phenylanthranilic acid did not affect the ultrastructure of the cells, suggested that plasma membrane chloride channels are involved in the initial signaling cascade triggered when lymphatic endothelial cells begin to adhere to a substrate.

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.