Chloride is required for receptor-mediated divalent cation entry in mesangial cells

Journal of Cellular Physiology
S G KremerK L Skorecki

Abstract

Agonists which stimulate the inositol 1,4,5 trisphosphate ([1,4,5]-IP3)-dependent mobilization of Ca2+ from intracellular stores also stimulate entry of divalent cations across the cell membrane. Under appropriate experimental conditions, divalent cation entry across the cell membrane can be monitored as the rate at which the intracellular fluorescence of divalent cation indicators is quenched by the addition of Mn2+ to the extracellular medium. We report that addition of vasopressin to fura-2-loaded glomerular mesangial cells in culture markedly accelerated the rate at which Mn2+ quenched fura-2 fluorescence at its Ca(2+)-insensitive wavelength in the presence of extracellular NaCl, but that this quench response was attenuated when Cl- was removed from the extracellular medium by equimolar substitution with impermeant anions (gluconate, methanesulfonate, acetate, lactate). Similarly, loss of agonist-induced quench also occurred when Cl- was substituted with gluconate in K(+)-containing media. Addition of the Cl- channel inhibitor, 5-nitro-2-(3-phenylpropylaminobenzoic acid) (NPPB), also inhibited Mn(2+)-induced quench of fura-2 fluorescence following vasopressin addition. In contrast, in the presence of gramicidin to provide a...Continue Reading

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Citations

Aug 1, 1995·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·N HackK L Skorecki
Jul 20, 1999·Kidney International·J GloyH Pavenstädt
Feb 14, 1998·Hypertension·A SpaldingW Lockette
Apr 3, 2015·Physiological Reviews·Mattias CarlströmWilliam J Arendshorst
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Aug 4, 1998·The American Journal of Physiology·L Balsam, N Nikbakht
Oct 12, 1999·The American Journal of Physiology·X Wang, C van Breemen
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Jul 23, 1998·Physiological Reviews·J D Stockand, S C Sansom
Mar 27, 2003·American Journal of Physiology. Renal Physiology·Randall K Wetzel, Kathleen J Sweadner

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