PMID: 3746250Aug 1, 1986Paper

Chloride movements in human neutrophils. Diffusion, exchange, and active transport

The Journal of General Physiology
L Simchowitz, P De Weer

Abstract

Chloride content and fluxes were measured in isolated resting human peripheral polymorphonuclear leukocytes. The intracellular Cl concentration of cells kept at 37 degrees C in 148 mM Cl media was approximately 80 meq/liter cell water, fourfold higher than expected for passive distribution at the cell's estimated membrane potential (approximately -53 mV). All intracellular Cl was rapidly exchangeable with external 36Cl. Cells lost Cl exponentially into Cl-free media, and reaccumulated it when Cl was restored to the bath; this reuptake was dependent on metabolism. One-way 36Cl fluxes in steady state cells were approximately 1.4 meq/liter X min. The bulk (approximately 70%) of these represented electrically silent Cl/Cl exchange mediated by a carrier insensitive to disulfonic stilbenes but blocked by the anion carrier inhibitor alpha-cyano-4-hydroxycinnamate (CHC). The remaining fluxes were characterized in some detail. About 20% of 36Cl influx behaved as active transport: it moved thermodynamically uphill and was absent in cells treated with 2-deoxy-D-glucose, displayed Michaelis-Menten kinetics with Km(Cl) congruent to 5 mM, Vmax congruent to 0.25 meq/liter X min, and was inhibited by CHC (Ki congruent to 1.7 mM), ethacrynate (...Continue Reading

Citations

Mar 21, 2008·Journal of Leukocyte Biology·Richard G PainterGuoshun Wang
Oct 9, 2008·The Journal of Biological Chemistry·A Paige Davis VolkJessica G Moreland
Feb 12, 2009·Antioxidants & Redox Signaling·Fred S LambFrancis J Miller
Jan 22, 2010·Journal of Leukocyte Biology·Richard G PainterGuoshun Wang
Jan 1, 1993·The Journal of Membrane Biology·L Simchowitz, S K Vogt
Jun 18, 2002·Journal of Neurochemistry·Lucien BettendorffPierre Wins
Aug 14, 2012·Antioxidants & Redox Signaling·Christine C Winterbourn, Anthony J Kettle
Nov 15, 1994·Annals of the New York Academy of Sciences·C N SerhanB D Levy
Jan 1, 1992·The European Journal of Neuroscience·G. Pocock, C. D. Richards
Sep 28, 2010·Free Radical Biology & Medicine·Ryan W BonvillainGuoshun Wang
Aug 30, 2008·Biochemical and Biophysical Research Communications·Jatinder Ahluwalia
Sep 14, 2007·Immunological Reviews·William M Nauseef
Feb 12, 1998·Biochimica Et Biophysica Acta·C Y ChangA Grider
Nov 27, 1987·Biochimica Et Biophysica Acta·C InceP C Leijh
Sep 1, 1988·Journal of Cellular Physiology·A C SherwoodM M Sanders
Feb 18, 2006·Biochimica Et Biophysica Acta·Ricardo Murphy, Thomas E DeCoursey
Sep 23, 2014·Free Radical Biology & Medicine·Jessie N GreenChristine C Winterbourn
Oct 24, 2012·Free Radical Biology & Medicine·Martha L AikenGuoshun Wang
May 9, 2001·Biochimica Et Biophysica Acta·M R MorrisM B Hallett
Mar 7, 2009·Biochemical and Biophysical Research Communications·Michael D Salmon, Jatinder Ahluwalia
Jun 17, 2016·Anesthesia and Analgesia·Koichi Yuki, Roderic G Eckenhoff
Sep 24, 2009·Chemico-biological Interactions·Rosita GabbianelliMasayasu Inoue
Aug 26, 2016·Immunological Reviews·Guoshun Wang
Sep 1, 1994·Metabolic Brain Disease·L Bettendorff
Nov 1, 2006·The Journal of Biological Chemistry·Christine C WinterbournAnthony J Kettle
Mar 20, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Chang-Won HongDong-Keun Song
Jun 7, 2015·Journal of Leukocyte Biology·Guoshun Wang, William M Nauseef
May 22, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Patricia Perez-CornejoPhilip A Knauf
Oct 18, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·R MenegazziP Patriarca
Jul 11, 1993·The American Journal of Physiology·L SimchowitzE J Cragoe
Jul 1, 1993·The American Journal of Physiology·J S StoddardL Simchowitz
Mar 16, 2017·Frontiers in Pharmacology·Juliet R FooteAnthony W Segal
Sep 6, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sara BusettoRenzo Menegazzi
Jun 1, 1990·The American Journal of Physiology·J J Garcia-Soto, S Grinstein
Feb 1, 1989·The American Journal of Physiology·M J MasonS Grinstein

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.