Chloride-stimulated sulfate efflux in Ehrlich ascites tumor cells: evidence for 1:1 coupling

Journal of Cellular Physiology
M L Villereal, C Levinson

Abstract

The kinetics of Cl-SO4-(2) exchange in Ehrlich ascites tumor cells was investigated in an attempt to determine the stoichiometry of this process. When tumor cells, equilibrated in Cl--free, 25 mM SO4-(2) medium are placed in SO4-(2)-free, 25 mm Cl-medium, both the net amount and rate of Cl-uptake far exceeds SO4-(2) loss.. Addition of the anion transport inhibitor SITS (4-acetamido-4,-isothiocyano-stilbene-2,2'-disulfonic acid) greatly reduces sulfate efflux (97%), but has no measurable effect on chloride uptake. Addition of furosemide, a Cl-transport inhibitor, reduces chloride uptake 94% but is without effect on sulfate efflux. These findings suggest that a chloride permeability pathway exists distinct from that utilized by SO4-(2). SITS, when added to furosemide treated cells, further reduces chloride uptake as well as inhibiting sulfate efflux, and under these experimental conditions, a linear relationship exists between SITS-sensitive, net chloride uptake and sulfate loss. The slope of this line is 1.05 (correlation coefficient = 0.996) which suggests the stoichiometry of Cl-SO4-(2) exchange is 1:1. Assuming a 1:1 stoichiometry, measurement of the initial chloride influx and initial sulfate efflux indicate that 92% of net ...Continue Reading

References

Feb 1, 1975·Journal of Cellular Physiology·C Levinson, M L Villereal
Jul 1, 1973·The American Journal of Physiology·M BurgN Green
Dec 1, 1973·Journal of Cellular Physiology·C Levinson, M L Villereal
Jan 1, 1974·The Journal of Membrane Biology·P A Fortes, J F Hoffman

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Citations

Jan 1, 1985·The Journal of Membrane Biology·C Levinson
Jan 1, 1981·The Journal of Membrane Biology·J E Langridge-Smith, M Field
Jan 1, 1988·Comparative Biochemistry and Physiology. A, Comparative Physiology·E H Larsen, K Simonsen
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·J B Pritchard, J L Renfro
Mar 10, 2001·The Journal of Experimental Zoology·G A GerencserM A Cattey
Oct 17, 1984·Biochimica Et Biophysica Acta·J E Langridge-SmithW P Dubinsky
Feb 27, 1986·Biochimica Et Biophysica Acta·E W Wolpaw, D L Martin
Jun 12, 1986·Biochimica Et Biophysica Acta·E K Hoffmann
Jan 16, 1989·Biochimica Et Biophysica Acta·T C Smith, C Levinson

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