Chloroacetaldehyde-induced mutagenesis in Escherichia coli: the role of AlkB protein in repair of 3,N(4)-ethenocytosine and 3,N(4)-alpha-hydroxyethanocytosine

Mutation Research
Agnieszka MaciejewskaJarosław T Kuśmierek

Abstract

Etheno (epsilon) adducts are formed in reaction of DNA bases with various environmental carcinogens and endogenously created products of lipid peroxidation. Chloroacetaldehyde (CAA), a metabolite of carcinogen vinyl chloride, is routinely used to generate epsilon-adducts. We studied the role of AlkB, along with AlkA and Mug proteins, all engaged in repair of epsilon-adducts, in CAA-induced mutagenesis. The test system used involved pIF102 and pIF104 plasmids bearing the lactose operon of CC102 or CC104 origin (Cupples and Miller (1989) [17]) which allowed to monitor Lac(+) revertants, the latter arose by GC-->AT or GC-->TA substitutions, respectively, as a result of modification of guanine and cytosine. The plasmids were CAA-damaged in vitro and replicated in Escherichia coli of various genetic backgrounds. To modify the levels of AlkA and AlkB proteins, mutagenesis was studied in E. coli cells induced or not in adaptive response. Formation of varepsilonC proceeds via a relatively stable intermediate, 3,N(4)-alpha-hydroxyethanocytosine (HEC), which allowed to compare repair of both adducts. The results indicate that all three genes, alkA, alkB and microg, are engaged in alleviation of CAA-induced mutagenesis. The frequency of m...Continue Reading

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Citations

Nov 14, 2012·The Journal of Biological Chemistry·Agnieszka M MaciejewskaJaroslaw T Kusmierek
Feb 1, 2012·PloS One·Damian MieleckiElżbieta Grzesiuk
Jan 5, 2011·Mutagenesis·Agnieszka M MaciejewskaJarosław T Kuśmierek
Aug 26, 2014·Free Radical Biology & Medicine·Masanori GotoHaruhiko Sugimura
Dec 3, 2016·Free Radical Biology & Medicine·Barbara TudekElżbieta Speina
Mar 4, 2014·Chemical Reviews·Guanqun ZhengChuan He
Jan 20, 2012·Free Radical Research·Alicja WinczuraBarbara Tudek
Nov 28, 2013·Environmental and Molecular Mutagenesis·Philip J Brooks, Samir Zakhari
May 9, 2014·FEMS Microbiology Letters·Damian Mielecki, Elżbieta Grzesiuk
Sep 15, 2019·Scientific Reports·Tomaš PilžysElżbieta Grzesiuk
Jul 15, 2015·The Journal of Biological Chemistry·Bogdan I FedelesJohn M Essigmann
Feb 23, 2018·Frontiers in Microbiology·Marike PalmerStephanus N Venter
Apr 15, 2017·The Biochemical Journal·Małgorzata DylewskaAgnieszka M Maciejewska
Mar 13, 2019·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Mark M MuenterPenny J Beuning
Sep 30, 2021·Physical Chemistry Chemical Physics : PCCP·Madison B BergerG Andrés Cisneros

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