Chlorogenic acid attenuates glucotoxicity in H9c2 cells via inhibition of glycation and PKC α upregulation and safeguarding innate antioxidant status

Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
M R Preetha RaniK G Raghu

Abstract

A series of cardiovascular complications associated with hyperglycemia is a critical threat to the diabetic population. Here we elucidate the link between hyperglycemia and cardiovascular diseases onset, focusing on oxidative stress and associated cardiac dysfunctions. The contribution of advanced glycation end products (AGE) and protein kinase C (PKC) signaling is extensively studied. For induction of hyperglycemia, H9c2 cells were incubated with 33 mM glucose for 48 h to simulate the diabetic condition in in vitro system. Development of cardiac dysfunction was confirmed with the significant increase of lactate dehydrogenase (LDH) release to the medium and associated decrease in cell viability. Various parameters like free radical generation, alteration in innate antioxidant system, lipid peroxidation, AGE production and PKC α -ERK axis were investigated during hyperglycemia and with chlorogenic acid. Hyperglycemia has significantly enhanced reactive oxygen species (ROS- 4 fold) generation, depleted SOD activity (1.3 fold) and expression of enzymes particularly CuZnSOD (SOD1) and MnSOD (SOD2), increased production of AGE (2.18 fold). Besides, PKC α dependent ERK signaling pathway was found activated (1.43 fold) leading to card...Continue Reading

References

Sep 15, 1989·Biochemical and Biophysical Research Communications·A A von RueckerF Bidlingmaier
May 1, 1987·European Heart Journal·H RuskoahoT Unger
Feb 1, 1993·Metabolism: Clinical and Experimental·B A ClarkD Elahi
Nov 24, 1999·Free Radical Biology & Medicine·H Sies
Apr 6, 2000·American Journal of Physiology. Renal Physiology·V ScivittaroM F Weiss
Apr 14, 2000·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·H KasaiH Mori
Sep 6, 2000·Diabetic Medicine : a Journal of the British Diabetic Association·K McKennaC J Thompson
Sep 16, 2000·Diabetologia·A I VinikG L Pittenger
Aug 7, 2001·Current Atherosclerosis Reports·W C Duckworth
Jan 5, 2002·Expert Opinion on Investigational Drugs·R A Kowluru, A Kennedy
Feb 5, 2002·Annual Review of Physiology·Gerald W Dorn, Daria Mochly-Rosen
Mar 5, 2003·Journal of Biochemical and Molecular Toxicology·A C MaritimJ B Watkins
May 29, 2003·The Journal of Nutrition·Marie-Paule GonthierAugustin Scalbert
Jul 6, 2006·Clinical and Experimental Hypertension : CHE·Takuya WatanabeIkuo Saito
Aug 4, 2007·Kidney International. Supplement·E Schleicher, U Friess
Jan 30, 2008·Free Radical Biology & Medicine·Siu-Wai ChoiBernadette M Hannigan
Aug 30, 2008·The American Journal of Medicine·Ashish AnejaMichael E Farkouh
Dec 17, 2009·Diabetes·Markus A QueisserKlaus T Preissner
Feb 9, 2010·Antioxidants & Redox Signaling·Carlotta GiorgiPaolo Pinton
Feb 26, 2010·Mediators of Inflammation·Hideaki KanetoTaka-aki Matsuoka
Oct 30, 2010·Circulation Research·Ferdinando Giacco, Michael Brownlee
Dec 25, 2010·Journal of Cardiovascular Pharmacology and Therapeutics·Richard Qinxue DingWei Zhou
Apr 9, 2011·Antioxidants & Redox Signaling·Tohru Fukai, Masuko Ushio-Fukai
Sep 29, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Hiroyuki TsutsuiShouji Matsushima
Feb 14, 2013·Diabetes Care·Chanshin ParkJonathan M Samet
Sep 26, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Shengxi MengYiyang Hu
Dec 5, 2013·Oxidative Medicine and Cellular Longevity·Kota V RamanaSharad S Singhal
Nov 5, 2014·Therapeutic Advances in Chronic Disease·Chris R Triggle, Hong Ding
Dec 30, 2014·Critical Reviews in Clinical Laboratory Sciences·Andreja TrpkovicEsma R Isenovic
Aug 4, 2015·Redox Biology·Cristina Espinosa-DiezSantiago Lamas

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiovascular Disease Pathophysiology

Cardiovascular disease involves several different processes that contribute to the pathological mechanism, including hyperglycemia, inflammation, atherosclerosis, hypertension and more. Vasculature stability plays a critical role in the development of the disease. Discover the latest research on cardiovascular disease pathophysiology here.

Cardiovascular Disorder in Diabetes

Diabetes is associated with an increased risk of cardiovascular disorders and heart failure. Discover the latest research here.

Cardiomyopathy

Cardiomyopathy is a disease of the heart muscle, that can lead to muscular or electrical dysfunction of the heart. It is often an irreversible disease that is associated with a poor prognosis. There are different causes and classifications of cardiomyopathies. Here are the latest discoveries pertaining to this disease.