Chlorophyll fluorescence screening of Arabidopsis thaliana for CO2 sensitive photorespiration and photoinhibition mutants

Functional Plant Biology : FPB
Murray R BadgerShunichi Takahashi

Abstract

Exposure of Arabidopsis thaliana (L.) photorespiration mutants to air leads to a rapid decline in the Fv/Fm chlorophyll fluorescence parameter, reflecting a decline in PSII function and an onset of photoinhibition. This paper demonstrates that chlorophyll fluorescence imaging of Fv/Fm can be used as an easy and efficient means of detecting Arabidopsis mutants that are impaired in various aspects of photorespiration. This screen was developed to be sensitive and high throughput by the use of exposure to zero CO2 conditions and the use of array grids of 1-week-old Arabidopsis seedlings as the starting material for imaging. Using this procedure, we screened ~25 000 chemically mutagenised M2 Arabidopsis seeds and recovered photorespiration phenotypes (reduction in Fv/Fm at low CO2) at a frequency of ~4 per 1000 seeds. In addition, we also recovered mutants that showed reduced Fv/Fm at high CO2. Of this group, we detected a novel 'reverse photorespiration' phenotype that showed a high CO2 dependent reduction in Fv/Fm. This chlorophyll fluorescence screening technique promises to reveal novel mutants associated with photorespiration and photoinhibition.

References

Apr 24, 2003·Journal of Experimental Botany·M A J ParryA J Keys
Jul 31, 2003·The Plant Journal : for Cell and Molecular Biology·Petra RennéAndreas P M Weber
Dec 13, 2005·Plant Physiology·Lars M VollAndreas P M Weber
Mar 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·S C Somerville, W L Ogren
Nov 4, 2006·Plant, Cell & Environment·Stephen P LongDonald R Ort
Apr 18, 2008·Journal of Experimental Botany·Inger Andersson
Jul 7, 2009·Annual Review of Plant Biology·Christine H FoyerGraham Noctor
Apr 1, 1988·Photosynthesis Research·R D BlackwellR M Wallsgrove

❮ Previous
Next ❯

Citations

Jun 3, 2016·Photosynthesis Research·Berkley J WalkerDonald R Ort
Feb 21, 2013·Plant, Cell & Environment·Qiying ZhouGuangcun He
Sep 18, 2013·Journal of Experimental Botany·L McAuslandT Lawson
Oct 11, 2017·Plant & Cell Physiology·John R EvansSusanne von Caemmerer
Aug 6, 2013·Journal of Experimental Botany·E H Murchie, T Lawson
Jan 14, 2017·Frontiers in Plant Science·Ivan SimkoRobert T Furbank
Oct 27, 2015·BMC Genomics·Eve KaurilindMikael Brosché
Mar 10, 2021·Journal of Plant Research·Takako Ogawa, Kintake Sonoike

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved