Chlorophyll-protein organization of membranes from the cyanobacterium Anacystis nidulans

Archives of Biochemistry and Biophysics
J A Guikema, L A Sherman

Abstract

Six chlorophyll-containing bands were observed upon electrophoretic analysis of Anacystis nidulans thylakoid membranes. These ranged in apparent molecular weights from approximately 360 to 45 kdalton. Measurements of the light absorption and chlorophyll fluorescence properties of these bands revealed numerous differences among the aggregates. The larger chlorophyll-protein complexes had a chlorophyll absorption maximum at 676 nm while the smallest band, band VI, at approximately 45 kdalton, absorbed at 668 nm. The chlorophyll-protein organization of four submembrane particles was also examined. Digitonin and N-tetradecyl-N,N-dimethyl-3-ammonio-1-pro-panesulfonate were used to fractionate thylakoids and each treatment yielded two green fractions after sucrose density gradient centrifugation. The upper green fractions of both procedures were enriched in band VI. In addition, these fractions showed low temperature fluorescence emission at 686 nm. Conversely, the lower green fractions were enriched in the larger bands (bands I and II), and yielded fluorescence emission at 696 and 716 nm. The gel electrophoresis analysis of these chlorophyll-protein bands revealed 11 peptides ranging in size from less than 10 to 64 kdaltons. The lar...Continue Reading

References

Aug 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·W L Butler, R J Strasser
Jul 8, 1975·Biochimica Et Biophysica Acta·W L Butler, M Kitajima
Jan 8, 1981·Biochimica Et Biophysica Acta·D A Sadewasser, L A Sherman
Aug 1, 1980·Journal of Bioenergetics and Biomembranes·J A Guikema, L A Sherman
Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·P Delepelaire, N H Chua
Feb 1, 1980·Plant Physiology·M Rusckowski, B A Zilinskas
May 1, 1980·Plant Physiology·J E MulletC J Arntzen
May 1, 1980·Plant Physiology·J E MulletC J Arntzen

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Citations

Aug 1, 1986·Journal of Bioenergetics and Biomembranes·G S Bullerjahn, L A Sherman
Mar 22, 2007·Photosynthesis Research·Abhay K Singh, Louis A Sherman
Nov 27, 1985·Biochimica Et Biophysica Acta·G S BullerjahnL A Sherman
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·H B PakrasiL A Sherman
Jan 1, 1984·Plant Physiology·J A Guikema, L A Sherman
Nov 6, 2008·Annual Review of Genetics·Stephan EberhardFrancis-André Wollman
Nov 1, 2001·Proceedings of the National Academy of Sciences of the United States of America·E ZakH B Pakrasi
Sep 14, 2006·Photosynthesis Research·Sofía AndaluzAnunciación Abadía
Nov 15, 1984·Archives of Biochemistry and Biophysics·T M Bricker, L A Sherman
Apr 1, 1984·Journal of Bacteriology·S S Golden, L A Sherman
Sep 1, 1983·Journal of Bacteriology·S S Golden, L A Sherman

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