Abstract
Chlorophyllin (CHLN) is a synthetic derivative of chlorophyll that possesses antimutagenic activity against several environmental contaminants. In the present study, CHLN was assayed for its capacity to prevent nuclear fragmentation (NF) in HEp-2 cells infected with poliovirus. CHLN was assayed at concentrations of 0.5 and 2.5 microg ml(-1), and NF was monitored using the comet assay and acridine orange staining. We demonstrated that CHLN reduced the percentage of NF in poliovirus-infected HEp-2 cells, when cells were treated with drug before infection or exposed continuously to drug. However, the highest degree of protection was achieved when the virus was exposed to CHLN before infection followed by protocol where infected cultures were continuously exposed to the drug after infection. It is suggested that CHLN primarily binds to the virus which inhibits cell penetration, thereby maintaining nuclear integrity. Considering that CHLN has several beneficial properties and no significant toxic effects in humans and animals, it would be an ideal candidate drug to test for antiviral activity.
References
Jan 1, 1990·Mutation Research·M D WatersH E Brockman
Jan 1, 1989·Carcinogenesis·T NegishiH Hayatsu
Jul 16, 1994·Mutation Research·N TachinoR Dashwood
Dec 1, 1994·Mutation Research·D SarkarG Talukder
Feb 1, 1995·Journal of Virology·E A TolskayaV I Agol
Jun 1, 1993·Mutation Research·S ArimotoH Hayatsu
Feb 1, 1995·Mutation Research·D W FairbairnK L O'Neill
Dec 1, 1995·Mutation Research·S ArimotoH Hayatsu
Feb 19, 1996·Mutation Research·S KnasmüllerH Bartsch
Jan 1, 1996·Mutagenesis·G C Yen, H Y Chen
Aug 8, 1996·Mutation Research·D SarkarG Talukder
May 12, 1997·Mutation Research·T NegishiH Hayatsu
Aug 1, 1997·Mutation Research·A P Odin
Jul 24, 1998·Mutation Research·H Nishino
Jun 15, 1999·Mutation Research·A N Vijaya LakshmiG Sharma
Aug 12, 1999·Methods in Molecular Biology·G Speit, A Hartmann
Dec 13, 2000·Mutation Research·E PimentelS Zimmering
Citations
Aug 19, 2014·Toxicological Sciences : an Official Journal of the Society of Toxicology·Akmal A El-GhorHanan Ramadan H Mohamed
Oct 13, 2014·Food Chemistry·Ana-Marija DomijanVera Garaj-Vrhovac
Jan 31, 2006·Letters in Applied Microbiology·R ArshadS S Ali
Oct 22, 2009·Letters in Applied Microbiology·F J BenatiC Nozawa
Apr 22, 2016·Toxicology Mechanisms and Methods·Gláucia Fernanda Rocha D EpiroMário Sérgio Mantovani
Feb 6, 2007·Archives of Virology·Y Kamei, M Aoki
May 1, 2021·Pharmaceuticals·Tapan BehlDomenico Montesano