Chloroplast ATP Synthase Modulation of the Thylakoid Proton Motive Force: Implications for Photosystem I and Photosystem II Photoprotection

Frontiers in Plant Science
Atsuko KanazawaDavid M Kramer

Abstract

In wild type plants, decreasing CO2 lowers the activity of the chloroplast ATP synthase, slowing proton efflux from the thylakoid lumen resulting in buildup of thylakoid proton motive force (pmf). The resulting acidification of the lumen regulates both light harvesting, via the qE mechanism, and photosynthetic electron transfer through the cytochrome b6f complex. Here, we show that the cfq mutant of Arabidopsis, harboring single point mutation in its γ-subunit of the chloroplast ATP synthase, increases the specific activity of the ATP synthase and disables its down-regulation under low CO2. The increased thylakoid proton conductivity (gH(+)) in cfq results in decreased pmf and lumen acidification, preventing full activation of qE and more rapid electron transfer through the b6f complex, particularly under low CO2 and fluctuating light. These conditions favor the accumulation of electrons on the acceptor side of PSI, and result in severe loss of PSI activity. Comparing the current results with previous work on the pgr5 mutant suggests a general mechanism where increased PSI photodamage in both mutants is caused by loss of pmf, rather than inhibition of CEF per se. Overall, our results support a critical role for ATP synthase reg...Continue Reading

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Citations

Apr 1, 2018·Physiologia Plantarum·Daisuke Takagi, Chikahiro Miyake
Aug 24, 2017·Angewandte Chemie·Youqian XuJunbai Li
Jan 14, 2020·Chembiochem : a European Journal of Chemical Biology·Lisa GerlandHartmut Oschkinat
May 6, 2020·The New Phytologist·Mattia StortiTomas Morosinotto
Jul 30, 2018·Photosynthesis Research·Wei HuangShi-Bao Zhang
Aug 14, 2019·Frontiers in Plant Science·Shin-Nosuke Hashida, Maki Kawai-Yamada
Feb 23, 2020·Frontiers in Plant Science·Geoffry A Davis, David M Kramer
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Jun 28, 2019·Plant Direct·Lauri NikkanenEevi Rintamäki
Nov 22, 2018·Frontiers in Plant Science·Ginga Shimakawa, Chikahiro Miyake
Sep 16, 2020·Journal of Experimental Botany·Anders K NilssonBjörn Lundin Burmeister
Nov 30, 2019·Plant Science : an International Journal of Experimental Plant Biology·Seon-Kap HwangThomas W Okita
Feb 18, 2021·Plant Molecular Biology·Gyedre Dos Santos AraújoEnéas Gomes-Filho
Jun 3, 2021·International Journal of Molecular Sciences·Violeta VelikovaSashka Krumova
Jun 26, 2021·Journal of Plant Research·Jeffrey A Cruz, Thomas J Avenson
Jul 1, 2021·Plant, Cell & Environment·Nícolas Zendonadi Dos SantosOnno Muller
Sep 8, 2021·Frontiers in Plant Science·Ginga ShimakawaChikahiro Miyake
Dec 21, 2021·Frontiers in Plant Science·Yugo Lima-MeloPeter J Gollan

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environmental stresses
electrophoresis

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ATP Synthases

ATP synthases are enzymes located in the inner mitochondrial membrane that catalyze the synthesis of ATP during cellular respiration. Discover the latest research on ATP synthases here.