PMID: 43141Oct 15, 1979

Chloroplast cytochrome b-563, hydrophobic environment and lack of direct reaction with ferredoxin

The Biochemical Journal
R P Cox

Abstract

1. The reaction of electron donors with cytochrome b-563 in chloroplasts was studied by investigating their effects on the rate of its reduction in the presence of dithionite, which reacts only slowly with the cytochrome. The relative effects of 9,10-anthraquinone and 9,10-anthraquinone 2-sulphonate in the presence of dithionite suggested that the site of attack of redox reagents was protected behind a hydrophobic barrier from the external medium. 2. Ferredoxin had no measurable effect on the rate of reduction of the cytochrome in the presence of dithionite. 3. The reduction of pigment P700 in the dark after illumination in the presence of the inhibitor 3-(3,4-dichlorophenyl)-1,1-dimethylurea was stimulated by a combination of NADPH and ferredoxin, although NADPH alone had little effect. The same combination was unable to reduce cytochrome b-563 at a measurable rate. 4. It is concluded that the cytochrome is unlikely to be part of a linear pathway of electron flow between ferredoxin and pigment P700.

References

Jan 20, 2016·Photosynthesis Research·Mathias LabsDario Leister
Jun 1, 1996·Annual Review of Plant Physiology and Plant Molecular Biology·W. A. CramerJ. L. Smith
Sep 1, 1988·Photosynthesis Research·D P O'Keefe

Related Concepts

Sodium Dithionite
Anthraquinones
Cytochromes
Spectrophotometry
NADP
Etioplasts
Oxidation-Reduction
Ferredoxin II

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Synapse Loss as Therapeutic Target in MS

As we age, the number of synapses present in the human brain starts to decline, but in neurodegenerative diseases this occurs at an accelerated rate. In MS, it has been shown that there is a reduction in synaptic density, which presents a potential target for treatment. Here is the latest research on synapse loss as a therapeutic target in MS.

Artificial Intelligence in Cardiac Imaging

Artificial intelligence (ai) techniques are increasingly applied to cardiovascular (cv) medicine in cardiac imaging analysis. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

Social Learning

Social learning involves learning new behaviors through observation, imitation and modeling. Follow this feed to stay up to date on the latest research.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Single Cell Chromatin Profiling

Techniques like ATAC-seq and CUT&Tag have the potential to allow single cell profiling of chromatin accessibility, histones, and TFs. This will provide novel insight into cellular heterogeneity and cell states. Discover the latest research on single cell chromatin profiling here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.