PMID: 5918715Sep 9, 1966

Chloroplast DNA from tobacco leaves

K K Tewari, S G Wildman


DNA from tobacco leaf chloroplasts was isolated as a single component with a buoyant density in CsCl of 1.702 compared to 1.697 for nuclear DNA. 5-Methylcytosine is present in nuclear DNA but absent in chloroplast DNA. Chloroplast DNA, with a guanine-cytosine content of 43 percent, has a melting temperature of 86 degrees C and renatures completely on slow cooling, whereas nuclear DNA ( melting temperature, 84 degrees C; guanine-cytosine content, 40 percent) does not renature. About 9 percent of the total DNA in tobacco leaves is chloroplast DNA representing about 4.7 xX 10-(15) gram of DNA per chloroplast with a molecular weight of approximately 4 xX 10(7).


Jan 1, 1988·Photosynthesis Research·A GnanamR M Mannan
Apr 1, 1983·Plant Cell Reports·R C ShoemakerR G Palmer
Jan 1, 1983·Human Genetics·D N Cooper
Sep 26, 1967·Biochimica Et Biophysica Acta·B Green, M P Gordon
Dec 16, 1969·Biochimica Et Biophysica Acta·S D Kung, J P Williams
May 19, 1976·Biochimica Et Biophysica Acta·M Bayen, J Dalmon
Jan 1, 1969·Progress in Biophysics and Molecular Biology·M R Pollock
Aug 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·K K Tewari, S G Wildman
Feb 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·K K Tewari, S G Wildman
Jun 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·A KadouriM Edelman
Jul 1, 1979·Plant Physiology·G K Lamppa, A J Bendich
Feb 1, 1980·Plant Physiology·D Bisaro, A Siegel
Mar 1, 1972·Plant Physiology·S D KungJ P Williams
Mar 4, 1976·Biochimica Et Biophysica Acta·J D Kemp, D W Sutton
Nov 20, 1968·Biochimica Et Biophysica Acta·S D Kung, J P Willisms
Nov 15, 1973·European Journal of Biochemistry·M Bayen, A Rode
Oct 24, 2013·The Plant Journal : for Cell and Molecular Biology·Lars B Scharff, Ralph Bock
Jul 1, 1968·Experimental Cell Research·J McLeish
Aug 1, 1981·Experimental Cell Research·T Kuroiwa, T Suzuki
Feb 14, 1968·Journal of Molecular Biology·C L Woodcock, H Fernández-Morán
Nov 1, 1967·Virology·A Hirai, S G Wildman
Oct 1, 1971·Virology·A Siegel
Jun 1, 1970·Virology·R J ShepherdR J Wakeman
Aug 23, 1967·Biochemical and Biophysical Research Communications·D Spencer, P R Whitfeld
Nov 14, 1971·Journal of Molecular Biology·K A Drlica, C A Knight
Jul 19, 1972·Nature: New Biology·J D Rochaix
Aug 1, 1967·Die Naturwissenschaften·A K Kleinschmidt
Oct 26, 1971·European Journal of Biochemistry·V Heilporn, S Limbosch

Related Concepts

DNA, Double-Stranded
Plants, Toxic
Nicotiana tabacum

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Pediculosis pubis

Pediculosis pubis is a disease caused by a parasitic insect known as Pthirus pubis, which infests human pubic hair, as well as other areas with hair including eye lashes. Here is the latest research.

Rh Isoimmunization

Rh isoimmunization is a potentially preventable condition that occasionally is associated with significant perinatal morbidity or mortality. Discover the latest research on Rh Isoimmunization here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells. It also follows CRISPR-Cas9 approaches to generating genetic mutants as a means of understanding the effect of genetics on phenotype.

Enzyme Evolution

This feed focuses on molecular models of enzyme evolution and new approaches (such as adaptive laboratory evolution) to metabolic engineering of microorganisms. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Pharmacology of Proteinopathies

This feed focuses on the pharmacology of proteinopathies - diseases in which proteins abnormally aggregate (i.e. Alzheimer’s, Parkinson’s, etc.). Discover the latest research in this field with this feed.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.