Chloroplast DNA Variations in Wild Brassicas and Their Implication in Breeding and Population Genetics Studies

Scientifica
Bharti SarinAparajita Mohanty

Abstract

Evaluation of chloroplast DNA (cpDNA) diversity in wild relatives of crop brassicas is important for characterization of cytoplasm and also for population genetics/phylogeographic analyses. The former is useful for breeding programs involving wide hybridization and synthesis of alloplasmic lines, while the latter is important for formulating conservation strategies. Therefore, PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) technique was applied to study cpDNA diversity in 14 wild brassicas (including 31 accessions) which revealed a total of 219 polymorphic fragments. The combination of polymorphisms obtained by using only two primer pair-restriction enzyme combinations was sufficient to distinguish all 14 wild brassicas. Moreover, 11 primer pairs-restriction enzyme combinations revealed intraspecific polymorphisms in eight wild brassicas (including endemic and endangered species, B. cretica and B. insularis, resp.). Thus, even within a small number of accessions that were screened, intraspecific polymorphisms were observed, which is important for population genetics analyses in wild brassicas and consequently for conservation studies.

References

Apr 1, 1997·Molecular Ecology·S Dumolin-LapegueR J Petit
Apr 3, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·S PandaI Aguinagalde
Dec 5, 2006·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·C J AllenderG J King
Feb 26, 2010·Journal of Experimental Botany·Laura A Chapman, Daphne R Goring
Feb 1, 1993·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·A M TorresA Martín
Nov 1, 1992·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·A K PradhanD Pental

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Methods Mentioned

BETA
Electrophoresis
PCR

Software Mentioned

pc
Image Lab TM
MXCOMP
NTSYS

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