Chloroplast-expressed MSI-99 in tobacco improves disease resistance and displays inhibitory effect against rice blast fungus

International Journal of Molecular Sciences
Yun-Peng WangShao-Chen Xing

Abstract

Rice blast is a major destructive fungal disease that poses a serious threat to rice production and the improvement of blast resistance is critical to rice breeding. The antimicrobial peptide MSI-99 has been suggested as an antimicrobial peptide conferring resistance to bacterial and fungal diseases. Here, a vector harboring the MSI-99 gene was constructed and introduced into the tobacco chloroplast genome via particle bombardment. Transformed plants were obtained and verified to be homoplastomic by PCR and Southern hybridization. In planta assays demonstrated that the transgenic tobacco plants displayed an enhanced resistance to the fungal disease. The evaluation of the antimicrobial activity revealed that the crude protein extracts from the transgenic plants manifested an antimicrobial activity against E. coli, even after incubation at 120 °C for 20 min, indicating significant heat stability of MSI-99. More importantly, the MSI-99-containing protein extracts were firstly proved in vitro and in vivo to display significant suppressive effects on two rice blast isolates. These findings provide a strong basis for the development of new biopesticides to combat rice blast.

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Citations

Dec 26, 2015·International Journal of Molecular Sciences·Yun-Peng WangShao-Chen Xing
Jun 5, 2016·Planta·Szymon Adam OlejniczakTomasz Sakowicz
Jun 25, 2016·Genome Biology·Henry DaniellWan-Jung Chang
Nov 5, 2016·Journal of Experimental Botany·Niaz AhmadPeter J Nixon
Oct 30, 2018·Protein and Peptide Letters·Rajeshwari Sinha, Pratyoosh Shukla
Apr 22, 2017·Plant Methods·Muhamed AdemTileye Feyissa
Jul 15, 2020·International Journal of Molecular Sciences·Yihe YuChoun-Sea Lin
Nov 23, 2020·Molecular Biology Reports·Ibrahim Ilker Ozyigit, Kuaybe Yucebilgili Kurtoglu

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Methods Mentioned

BETA
transgenic
PCR
enzyme-linked immunosorbent assay
ELISA
Assay

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