Chloroplast fructose-1,6-bisphosphatase: modification of non-covalent interactions promote the activation by chimeric Escherichia coli thioredoxins

FEBS Letters
S Mora-GarcíaR A Wolosiuk

Abstract

Although all thioredoxins contain a highly conserved amino acid sequence responsible for thiol/disulfide exchanges, only chloroplast thioredoxin-f is effective in the reductive stimulation of chloroplast fructose-1,6-bisphosphatase. We set out to determine whether Escherichia coli thioredoxin becomes functional when selected modulators alter the conformation of the target enzyme. Wild type and chimeric Escherichia coli thioredoxins match the chloroplast counterpart when the activation of chloroplast fructose 1,6-biphosphatase is performed in the presence of fructose 1,6-bisphosphate, Ca2+, and either trichloroacetate or 2-propanol. These modulators of enzyme activity do change the conformation of chloroplast fructose-1,6-bisphosphatase whereas bacterial thioredoxins remain unaltered. Given that fructose 1,6-bisphosphate, Ca2+, and non-physiological perturbants modify non-covalent interactions of the protein but do not participate in redox reactions, these results strongly suggest that the conformation of the target enzyme regulates the rate of thiol/disulfide exchanges catalyzed by protein disulfide oxidoreductases.

References

Jun 24, 1992·Biochimica Et Biophysica Acta·I HäberleinH Follmann
Mar 14, 1991·European Journal of Biochemistry·F de Lamotte-GueryJ P Jacquot
Sep 1, 1967·European Journal of Biochemistry·A Holmgren, P Reichard
Jan 1, 1984·Methods in Enzymology·D A HillsonR B Freedman
May 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R A WolosiukK Hagelin
Sep 1, 1996·Clinical and Experimental Pharmacology & Physiology·L F ArnoldaJ P Chalmers
Aug 21, 2013·Endocrinology·Christoffer ClemmensenKaren K Ryan

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Citations

Apr 22, 2014·Plant & Cell Physiology·Matías D HartmanAlberto A Iglesias
Jan 8, 2000·The Journal of Biological Chemistry·M A BallicoraJ Preiss
Apr 11, 2001·Biochimica Et Biophysica Acta·P D Duek, R A Wolosiuk

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