Chloroplast outer envelope protein CHUP1 is essential for chloroplast anchorage to the plasma membrane and chloroplast movement.

Plant Physiology
K OikawaMasamitsu Wada

Abstract

Chloroplasts change their intracellular distribution in response to light intensity. Previously, we isolated the chloroplast unusual positioning1 (chup1) mutant of Arabidopsis (Arabidopsis thaliana). This mutant is defective in normal chloroplast relocation movement and shows aggregation of chloroplasts at the bottom of palisade mesophyll cells. The isolated gene encodes a protein with an actin-binding motif. Here, we used biochemical analyses to determine the subcellular localization of full-length CHUP1 on the chloroplast outer envelope. A CHUP1-green fluorescent protein (GFP) fusion, which was detected at the outermost part of mesophyll cell chloroplasts, complemented the chup1 phenotype, but GFP-CHUP1, which was localized mainly in the cytosol, did not. Overexpression of the N-terminal hydrophobic region (NtHR) of CHUP1 fused with GFP (NtHR-GFP) induced a chup1-like phenotype, indicating a dominant-negative effect on chloroplast relocation movement. A similar pattern was found in chloroplast OUTER ENVELOPE PROTEIN7 (OEP7)-GFP transformants, and a protein containing OEP7 in place of NtHR complemented the mutant phenotype. Physiological analyses of transgenic Arabidopsis plants expressing truncated CHUP1 in a chup1 mutant bac...Continue Reading

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Citations

Jul 22, 2009·Proceedings of the National Academy of Sciences of the United States of America·Akeo KadotaMasamitsu Wada
Apr 27, 2010·Proceedings of the National Academy of Sciences of the United States of America·Noriyuki SuetsuguMasamitsu Wada
Oct 27, 2010·Proceedings of the National Academy of Sciences of the United States of America·Yutaka KodamaMasamitsu Wada
May 14, 2009·The Journal of Biological Chemistry·Juliette Jouhet, John C Gray
Jun 24, 2009·Journal of Experimental Botany·Shingo TakagiNami Sakurai-Ozato
Feb 18, 2010·Journal of Experimental Botany·Christopher J StaigerLaurent Blanchoin
Feb 9, 2012·Journal of Experimental Botany·Agnieszka Katarzyna BanaśHalina Gabryś
Sep 29, 2011·Plant & Cell Physiology·Hideyasu Takamatsu, Shingo Takagi
Mar 5, 2014·Proceedings of the National Academy of Sciences of the United States of America·Takeshi HigaMasamitsu Wada
Jan 30, 2014·Annual Review of Plant Biology·Katherine W Osteryoung, Kevin A Pyke
Feb 3, 2016·Frontiers in Plant Science·Kathleen DelfosseJaideep Mathur
Jan 23, 2016·Journal of Plant Research·Sam-Geun Kong, Masamitsu Wada
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Mar 11, 2011·Plant Signaling & Behavior·Yutaka KodamaMasamitsu Wada
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May 21, 2016·Frontiers in Plant Science·Noriyuki Suetsugu, Masamitsu Wada
Jul 16, 2013·Plant Science : an International Journal of Experimental Plant Biology·Masamitsu Wada

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