Chloroplast Protein 12 Expression Alters Growth and Chilling Tolerance in Tropical Forage Stylosanthes guianensis (Aublet) Sw

Frontiers in Plant Science
Kailong LiShaoyun Lu

Abstract

Stylosanthes guianensis (Aublet) Sw. is a tropical forage legume with soil acidity tolerance and excellent adaptation to infertile soils, but sensitive to chilling. To understand the molecular responses of S. guianensis to chilling, differentially expressed genes between a chilling tolerant mutant 7-1 and the wild type were identified using suppression subtractive hybridization, and eight of them were confirmed and the regulation pattern were analyzed using quantitative reverse transcription PCR (qRT-PCR). Chloroplast protein 12 (CP12) functions to regulate the Calvin cycle by forming a ternary complex with glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and phosphoribulokinase (PRK). SgCP12 transcript was induced by chilling in both plants, and higher levels were observed in 7-1 than in the wild type, implying a potential role of SgCP12 in chilling tolerance. To confirm this, transgenic S. guianensis plants over-expressing or down-regulating SgCP12 were generated, respectively. Higher Fv/Fm and survival rate and lower ion leakage were observed in transgenic plants overexpressing SgCP12 as compared with the wild type after chilling treatment, while lower Fv/Fm and survival rate and higher ion leakage were found in SgCP12 antis...Continue Reading

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Citations

Feb 18, 2019·Journal of Experimental Botany·Andrew J SimkinChristine A Raines
Oct 2, 2019·Proceedings of the National Academy of Sciences of the United States of America·Ciaran R McFarlaneJames W Murray
Mar 26, 2021·Cell Communication and Signaling : CCS·Hui ShaoHélène Launay

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Methods Mentioned

BETA
transgenic
Reverse Transcription PCR
PCR

Software Mentioned

SPSS
DNAMAN

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