Chloroplastic and cytoplasmic overexpression of sheep serotonin N-acetyltransferase in transgenic rice plants is associated with low melatonin production despite high enzyme activity

Journal of Pineal Research
Yeong ByeonKyoungwhan Back

Abstract

Serotonin N-acetyltransferase (SNAT), the penultimate enzyme in melatonin biosynthesis, catalyzes the conversion of serotonin into N-acetylserotonin. Plant SNAT is localized in chloroplasts. To test SNAT localization effects on melatonin synthesis, we generated transgenic rice plants overexpressing a sheep (Ovis aries) SNAT (OaSNAT) in their chloroplasts and compared melatonin biosynthesis with that of transgenic rice plants overexpressing OaSNAT in their cytoplasm. To localize the OaSNAT in chloroplasts, we used a chloroplast targeting sequence (CTS) from tobacco protoporphyrinogen IX oxidase (PPO), which expresses in chloroplasts. The purified recombinant CTS:OaSNAT fusion protein was enzymatically functional and localized in chloroplasts as confirmed by confocal microscopic analysis. The chloroplast-targeted CTS:OaSNAT lines and cytoplasm-expressed OaSNAT lines had similarly high SNAT enzyme activities. However, after cadmium and butafenacil treatments, melatonin production in rice leaves was severalfold lower in the CTS:OaSNAT lines than in the OaSNAT lines. Notably, enhanced SNAT enzyme activity was not directly proportional to the production of N-acetylserotonin, melatonin, or 2-hydroxymelatonin, suggesting that plant SNA...Continue Reading

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Feb 1, 2012·Journal of Pineal Research·Yeong ByeonKyoungwhan Back
May 30, 2012·Journal of Pineal Research·Sangkyu Park, Kyoungwhan Back
Aug 4, 2012·Journal of Pineal Research·Sangkyu ParkKyoungwhan Back
Oct 19, 2013·Journal of Pineal Research·Yeong ByeonKyoungwhan Back
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Nov 5, 2014·Journal of Pineal Research·Jerry Vriend, Russel J Reiter

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Citations

Sep 4, 2015·Journal of Pineal Research·Yeong ByeonKyoungwhan Back
Jun 23, 2015·Journal of Pineal Research·Marino B Arnao, Josefa Hernández-Ruiz
Nov 2, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Annia GalanoRussel J Reiter
Dec 22, 2016·International Journal of Molecular Sciences·Dun-Xian TanRussel J Reiter
Mar 3, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Russel J ReiterBing Xu
Mar 31, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jun NiLifang Wu
Mar 2, 2019·Environmental Science and Pollution Research International·Mustafa Okant, Cengiz Kaya

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