Chloroplasts of salt-grown Arabidopsis seedlings are impaired in structure, genome copy number and transcript levels

PloS One
Petra Peharec ŠtefanićDudy Bar-Zvi

Abstract

The chloroplast is the most prominent and metabolically active plastid in photosynthetic plants. Chloroplasts differentiate from proplastids in the plant meristem. Plant plastids contain multiple copies of a small circular genome. The numbers of chloroplasts per mesophyll cell and of plastid genome copies are affected by developmental stage and environmental signals. We compared chloroplast structure, gene expression and genome copy number in Arabidopsis seedlings germinated and grown under optimal conditions to those in seedlings germinated and grown in the presence of NaCl. Chloroplasts of the NaCl-grown seedlings were impaired, with less developed thylakoid and granum membranes than control seedlings. In addition, chloroplasts of salt-grown Arabidopsis seedlings accumulated more starch grains than those in the respective control plants. Steady-state transcript levels of chloroplast-encoded genes and of nuclear genes encoding chloroplast proteins were reduced in salt-grown seedlings. This reduction did not result from a global decrease in gene expression, since the expression of other nuclear genes was induced or not affected. Average cellular chloroplast genome copy number was reduced in salt-grown seedlings, suggesting that...Continue Reading

References

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Citations

May 5, 2017·Journal of Experimental Botany·Jayakumar BoseStephen D Tyerman
Jun 26, 2018·Plant, Cell & Environment·Julia A RichterChristian Zörb
Mar 3, 2019·International Journal of Molecular Sciences·Pedro Robles, Víctor Quesada
Aug 28, 2021·Cells·Abdul HameedBrent L Nielsen
Sep 1, 2021·Plant Physiology and Biochemistry : PPB·Hisham A ElshokyEmilia Apostolova

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Methods Mentioned

BETA
salt stress
electron microscopy
transmission electron microscopy

Software Mentioned

Primer
Express

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