Abstract
Antimalarials chloroquine (CQ) and hydroxychloroquine (HCQ) are widely used as antiinflammatory drugs, but side effects include retinopathy and vision loss. The objective of this study was to examine the effect of CQ and HCQ on the barrier integrity of retinal pigment epithelial (RPE) cell monolayers in vitro. Permeability of ARPE-19 cell monolayers was determined using Fluorescein isothiocyanate (FITC)-labeled dextran. The influence of CQ and HCQ on cell death and the expression tight junction molecules was examined. CQ and HCQ significantly increased ARPE-19 monolayer permeability after 3 and 18 h, respectively, and enhanced mRNA levels for claudin-1 and occludin. Cytotoxicity was only observed after 18 h exposure. Thus, CQ and HCQ rapidly enhance RPE barrier permeability in vitro, independent of cytotoxicity or loss of zonula occludens-1, claudin-1, and occludin expression. Our findings suggest that CQ/HCQ-induced permeability of the RPE layer may contribute to blood-retinal barrier breakdown in case of CQ/HCQ-induced retinopathy.
References
Jan 1, 1978·Scandinavian Journal of Rheumatology·F A WollheimC B Laurell
May 1, 1987·Arthritis and Rheumatism·D R MillerV J Stecher
May 1, 1967·Toxicology and Applied Pharmacology·E W McChesneyR J Fabian
Jul 18, 1983·The American Journal of Medicine·H N Bernstein
Jul 18, 1983·The American Journal of Medicine·R I Rynes
Jul 18, 1983·The American Journal of Medicine·A H Mackenzie
Oct 1, 1993·Seminars in Arthritis and Rheumatism·M Easterbrook
Mar 1, 1996·Annals of the Rheumatic Diseases·M MavrikakisK Rougas
Feb 3, 1999·Regulatory Toxicology and Pharmacology : RTP·B LeblancC Taradach
May 7, 2002·American Journal of Ophthalmology·David J Browning
Aug 24, 2002·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Staffan P Sundelin, Alexei Terman
Aug 27, 2003·Investigative Ophthalmology & Visual Science·Toshiaki AbeMakoto Tamai
Oct 3, 1959·Lancet·H E HOBBSA FREEDMAN
Jul 1, 2005·Physiological Reviews·Olaf Strauss
Feb 8, 2006·Annual Review of Physiology·Christina M Van Itallie, James M Anderson
Mar 7, 2007·International Review of Cytology·Lawrence J Rizzolo
Mar 29, 2007·Annals of the Rheumatic Diseases·Graciela S AlarcónUNKNOWN LUMINA Study Group
Jun 25, 2010·Investigative Ophthalmology & Visual Science·Young Hee YoonJae-Young Koh
Feb 5, 2011·Ophthalmology·Michael F MarmorUNKNOWN American Academy of Ophthalmology
Jun 30, 2011·Cell & Bioscience·Patrick M ChenJeff W Chen
Oct 8, 2011·Current Eye Research·Tadanobu YoshikawaKanji Takahashi
Jul 28, 2013·JAMA Ophthalmology·Mihai MititeluAmani A Fawzi
Dec 5, 2013·Investigative Ophthalmology & Visual Science·Jeroen BastiaansWillem A Dik
Citations
Oct 16, 2015·Ophthalmology·Vishal S ParikhRishi P Singh
Oct 3, 2015·Journal of Pharmaceutical Sciences·Chenghao XuFanfan Zhou
Jan 11, 2016·Annals of the New York Academy of Sciences·Madhu M OusephQing Jun Wang
Mar 11, 2017·Eye·I H YusufS M Downes
Nov 13, 2018·Journal of Cellular Physiology·Ting-Yu ChenChia-Yih Wang
Mar 8, 2020·Biomedicines·Alfonso CasadoAndrea Cerveró
Nov 9, 2019·Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft·A RickmannG Szurman
Aug 31, 2018·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Mehmet BulutHasan Fatih Çay
Nov 8, 2018·Nature Reviews. Rheumatology·April JorgeHyon K Choi
Aug 29, 2020·International Ophthalmology·Hatice Nur TarakciogluUlviye Yigit
Aug 3, 2020·Asia-Pacific Journal of Ophthalmology·Wanda Martinez NavarroAshutosh M Shukla
Mar 25, 2021·Cutaneous and Ocular Toxicology·Naciye KabataşCem Özişler
Apr 25, 2021·Rheumatology International·Romain Muller
Jun 5, 2021·Medicine·Gang WangJingyang Li
Aug 8, 2021·International Journal of Environmental Research and Public Health·Hung-Chih ChenJames Cheng-Chung Wei
Jul 30, 2020·Chemico-biological Interactions·Jia-Hui LiHong-Wei Pan
Jul 3, 2021·Journal of Biomaterials Applications·Baltej DhillonSudipta Seal