Chloroquine as a promising adjuvant therapy for type 1 Diabetes Mellitus.

Scientific Reports
Renato Ferreira de Almeida JúniorAdriana Augusto de Rezende

Abstract

Chloroquine (CQ) and hydroxychloroquine, are promising anti-inflammatory drugs for the treatment of Diabetes mellitus (DM) to prevent associated complications. Therefore, this study evaluated the anti-inflammatory effects of CQ-free and CQ-incorporated polylactic acid nanoparticles (NPs) in the peripheral blood mononuclear cells (PBMCs) of patients with type 1 Diabetes mellitus (T1DM). In total, 25 normoglycemic individuals and 25 patients with T1DM aged 10-16 years were selected and glycemic controls evaluated. After cell viability assessed by MTT assay, T1DM PBMCs were subjected to a CQ concentration of 10 µM in three different conditions: not treated (NT), treated with CQ, and treated with CQ NPs. The cells were incubated for 48 h, and the mRNA expressions of cytokines IL1B, IFNG, TNFA, IL12, and IL10 were determined by relative quantification through real-time PCR at 24 h intervals. IL1B expression decreased in CQ and CQ NP-treated cells after 48 h (p < 0.001) and 24 h (p < 0.05) of treatment, respectively. IFNG and IL12 expressions significantly decreased (p < 0.001) in cells treated with CQ and CQ NPs at 24 and 48 h compared to NT. TNFA and IL10 expressions significantly decreased after 48 h (p < 0.001) and 24 h (p < 0.00...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Aug 5, 2008·Molecular Pharmaceutics·Frank AlexisOmid C Farokhzad
Jan 12, 2011·Clinical Reviews in Allergy & Immunology·Ilan Ben-ZviYehuda Shoenfeld
Mar 15, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Alenka KuznikRoman Jerala
Feb 22, 2012·Trends in Molecular Medicine·James E TooleyKevan C Herold
Jun 7, 2013·Arthritis and Rheumatism·Stephen P YoungKarim Raza
Sep 4, 2013·Immunotherapy·Florian M P MeierUlf Müller-Ladner
Jan 7, 2015·International Journal of Molecular Sciences·Shivaprasad H VenkateshaKamal D Moudgil
Feb 13, 2015·Clinical Reviews in Allergy & Immunology·Nathalie Costedoat-ChalumeauMichael F Marmor
Apr 18, 2015·Indian Journal of Clinical Biochemistry : IJCB·N Maithili Karpaga SelviR Sripradha
Jul 22, 2015·Ageing Research Reviews·Jung Jae ShinWook Kim
Jul 12, 2016·Pharmaceuticals·Ioannis L LiakosAthanassia Athanassiou
Jul 22, 2016·Diabetes Care·Rena M PollackGil Leibowitz
Sep 30, 2016·Clinical Reviews in Allergy & Immunology·Ayelet KaminitzNadir Askenasy
Jan 31, 2017·Canadian Journal of Physiology and Pharmacology·Changyu DingJiang Zheng
Feb 9, 2017·Scientific Reports·Ralf L J SchmidtKlaus G Schmetterer
Mar 31, 2017·Nature Reviews. Disease Primers·Anastasia KatsarouÅke Lernmark
Oct 31, 2017·The Nursing Clinics of North America·Anne Kay Brinkman
May 3, 2018·Beilstein Journal of Nanotechnology·Jaison JeevanandamMichael K Danquah

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

GraphPad Prism
[UNK]
NORMFINDER
GraphPad

Related Concepts

Related Feeds

Antimalarial Agents (ASM)

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Antimalarial Agents

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Related Papers

Caring : National Association for Home Care Magazine
Cambridge Quarterly of Healthcare Ethics : CQ : the International Journal of Healthcare Ethics Committees
Cambridge Quarterly of Healthcare Ethics : CQ : the International Journal of Healthcare Ethics Committees
Cambridge Quarterly of Healthcare Ethics : CQ : the International Journal of Healthcare Ethics Committees
D A Buehler
Cambridge Quarterly of Healthcare Ethics : CQ : the International Journal of Healthcare Ethics Committees
© 2021 Meta ULC. All rights reserved