Chloroquine resistance is associated to multi-copy pvcrt-o gene in Plasmodium vivax malaria in the Brazilian Amazon

Malaria Journal
Siuhelem R SilvaGisely Cardoso de Melo

Abstract

The resistance of Plasmodium vivax to chloroquine has become an obstacle to control strategies based on the use of anti-malarials. The current study investigated the association between P. vivax CQ-resistance in vivo with copy number variation and mutations in the promoter region in pvcrt-o and pvmdr1 genes. The study included patients with P. vivax that received supervised treatment with chloroquine and primaquine. Recurrences were actively recorded during this period. Among the 60 patients with P. vivax, 25 were CQ-resistant and 35 CQ-susceptible. A frequency of 7.1% of multi-copy pvcrt-o was observed in CQ-susceptible samples and 7.7% in CQ-resistant at D0 (P > 0.05) and 33.3% in CQ-resistant at DR (P < 0.05). For pvmdr1, 10.7% of the CQ-susceptible samples presented multiple copies compared to 11.1% in CQ-resistant at D0 and 0.0% in CQ-resistant at DR (P > 0.05). A deletion of 19 bp was found in 11/23 (47.6%) of the patients with CQ-susceptible P. vivax and 3/10 (23.1%) of the samples with in CQRPv at D0. At day DR, 55.5% of the samples with CQRPv had the 19 bp deletion. For the pvmdr-1 gene, was no variation in the analysed gene compared to the P. vivax reference Sal-1. This was the first study with 42-day clinical follow-...Continue Reading

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Citations

Jun 4, 2019·PLoS Neglected Tropical Diseases·Lisa H VerzierJulian C Rayner
Sep 5, 2019·Antimicrobial Agents and Chemotherapy·Lise MussetFélix Djossou
Oct 28, 2019·Current Topics in Medicinal Chemistry·Kamilla Rodrigues RogerioNubia Boechat
May 24, 2019·Briefings in Functional Genomics·Annie N Cowell, Elizabeth A Winzeler
May 6, 2020·Antimicrobial Agents and Chemotherapy·Anne C G AlmeidaGisely C Melo
Jan 10, 2021·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Soheila AsaliShahram Solaymani-Mohammadi
Dec 29, 2020·International Journal for Parasitology. Drugs and Drug Resistance·Marcelo U FerreiraJosé Pedro Gil
May 7, 2021·International Journal for Parasitology. Drugs and Drug Resistance·Lucas E BuyonManoj T Duraisingh
Jun 3, 2021·International Journal of Environmental Research and Public Health·Antonio A S BalieiroDaniel A M Villela

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Methods Mentioned

BETA
PCR
genotyping

Software Mentioned

BLAST
CQRPv
Geneious
Gene Marker
SPSS

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