Chlorosis as a Developmental Program in Cyanobacteria: The Proteomic Fundament for Survival and Awakening.

Molecular & Cellular Proteomics : MCP
Philipp SpätKarl Forchhammer

Abstract

Cyanobacteria that do not fix atmospheric nitrogen gas survive prolonged periods of nitrogen starvation in a chlorotic, dormant state where cell growth and metabolism are arrested. Upon nutrient availability, these dormant cells return to vegetative growth within 2-3 days. This resuscitation process is highly orchestrated and relies on the stepwise reinstallation and activation of essential cellular structures and functions. We have been investigating the transition to chlorosis and the return to vegetative growth as a simple model of a cellular developmental process and a fundamental survival strategy in biology. In the present study, we used quantitative proteomics and phosphoproteomics to describe the proteomic landscape of a dormant cyanobacterium and its dynamics during the transition to vegetative growth. We identified intriguing alterations in the set of ribosomal proteins, in RuBisCO components, in the abundance of central regulators and predicted metabolic enzymes. We found O-phosphorylation as an abundant protein modification in the chlorotic state, specifically of metabolic enzymes and proteins involved in photosynthesis. Nondegraded phycobiliproteins were hyperphosphorylated in the chlorotic state. We provide eviden...Continue Reading

References

Aug 9, 1990·Biochimica Et Biophysica Acta·R de LorimierS E Stevens
Jan 1, 1969·Archiv für Mikrobiologie·M M Allen, A J Smith
Dec 1, 1994·Microbiology·N H Mann
Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·R Schwarz, A R Grossman
Apr 5, 2005·The Journal of Biological Chemistry·Irina PivenAnna Sokolenko
Jul 29, 2005·Photosynthesis Research·Elena G AndrizhiyevskayaJan P Dekker
Jan 18, 2006·The New Phytologist·Bertrand Hirel, André Gallais
Jan 18, 2006·Journal of Molecular Microbiology and Biotechnology·Cheng-Cai ZhangLi Wang
Nov 3, 2007·Journal of Experimental Botany·Urs FellerTadahiko Mae
Nov 21, 2007·International Journal of Biological Sciences·Xiangyu GuanXuexi Tang
Mar 21, 2009·Nature Protocols·Paul J BoersemaAlbert J R Heck
Apr 14, 2009·Nature Methods·Daniel G GibsonHamilton O Smith
Nov 3, 2009·Nucleic Acids Research·Mitsuteru NakaoYasukazu Nakamura
Jul 5, 2011·Proteomics·Boris Macek, Ivan Mijakovic
Dec 6, 2011·Annual Review of Plant Biology·Loïc RajjouDominique Job
Dec 4, 2012·Nucleic Acids Research·Juan Antonio VizcaínoHenning Hermjakob
Jan 24, 2013·Nature Communications·Iakov I DavydovMarina V Rodnina
Apr 18, 2013·Journal of Proteome Research·Nelson C SoaresBoris Macek
Jun 19, 2013·Cell Host & Microbe·Emily S C RittershausChristopher M Sassetti
May 1, 1996·World Journal of Microbiology & Biotechnology·B B SinghP S Bisen
Mar 22, 2014·Microbiology·Frédéric DeschoenmaekerRuddy Wattiez
Nov 2, 2014·Photosynthesis Research·Natalia BattchikovaEva-Mari Aro

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Citations

Oct 16, 2018·Environmental Microbiology·Karl Forchhammer, Rakefet Schwarz
Aug 19, 2018·Applied and Environmental Microbiology·Björn Watzer, Karl Forchhammer
Feb 3, 2021·Proceedings of the National Academy of Sciences of the United States of America·Tim OrthweinKarl Forchhammer
Jan 30, 2021·Proceedings of the National Academy of Sciences of the United States of America·Vanessa KrauspeWolfgang R Hess
Jun 23, 2021·Trends in Biotechnology·Amit Srivastava, Pratyoosh Shukla
Aug 25, 2021·MSystems·Maja SemanjskiBoris Macek
Nov 1, 2021·Plant & Cell Physiology·Akiko Yoshihara, Koichi Kobayashi

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