Chlorpyrifos induces apoptosis and DNA damage in Drosophila through generation of reactive oxygen species

Ecotoxicology and Environmental Safety
Subash Chandra GuptaDebapratim Kar Chowdhuri

Abstract

The present study investigated the apoptosis and DNA damage inducing potential of chlorpyrifos (CP) in Drosophila melanogaster. Third instar larvae of Drosophila were treated with different concentrations of CP (0.015-15.0 microg/L) for 2-48 h. Reactive oxygen species (ROS) generation, oxidative stress markers, DNA damage and apoptotic cell death end points were measured in them. A significant increase in DNA damage was concomitant with apoptotic mode of cell death in 15.0 microg/L CP-treated organisms for 24 and 48 h. Depolarization in mitochondrial membrane potential and increased casapase-3 and caspase-9 activities in these organisms indicated both as potential targets of CP. A significant positive correlation was observed among ROS generation, apoptosis and DNA damage. The study suggests that (i) ROS may be involved in inducing apoptosis and DNA damage in the CP-exposed larvae of Drosophila and (ii) D. melanogaster may be used as an alternative in vivo animal model for xenobiotics hazard assessment.

References

Jun 1, 1979·Analytical Biochemistry·H OhkawaK Yagi
Feb 1, 1992·Experimental Cell Research·P L OliveJ P Banáth
Jan 1, 1990·Methods in Enzymology·R L LevineE R Stadtman
Mar 1, 1988·Experimental Cell Research·N P SinghE L Schneider
Jan 31, 1972·Biochemical and Biophysical Research Communications·M NishikimiK Yagi
Jun 1, 1972·Analytical Biochemistry·A K Sinha
Feb 20, 1998·Archives of Environmental Contamination and Toxicology·M T MooreC M Cooper
Jun 2, 1998·Drug Metabolism Reviews·S Linn
Sep 11, 1998·Archives of Environmental Contamination and Toxicology·S J Gruber, M D Munn
Oct 6, 1999·Archives of Environmental Contamination and Toxicology·M J LydyM A Ternes
Oct 13, 2001·Archives of Environmental Contamination and Toxicology·A NazirD Kar Chowdhuri
May 23, 2002·Biochimie·Christophe FleuryJean-Luc Vayssière
Jan 1, 2003·Archives of Environmental Health·Jack D ThrasherAlan Broughton
Apr 18, 2003·Frontiers in Bioscience : a Journal and Virtual Library·David M WilsonDaniel R McNeill
Jun 5, 2003·Neurotoxicology·Helmut V B HirschDouglas M Ruden
May 1, 1959·Archives of Biochemistry and Biophysics·G L ELLMAN
Jan 7, 2004·Mechanisms of Ageing and Development·Naoaki IshiiSatoru Furukawa
Dec 29, 2004·Free Radical Research·Marcela S VillaverdeSandra V Verstraeten
May 7, 2005·Archaea : an International Microbiological Journal·Tim Soderberg
Aug 23, 2005·Environmental and Molecular Mutagenesis·Hifzur R SiddiqueD Kar Chowdhuri
Jun 22, 2006·Toxicology·Ari NakadaiTomoyuki Kawada
Sep 19, 2006·The International Journal of Biochemistry & Cell Biology·Marian ValkoJoshua Telser
Oct 5, 2006·Current Alzheimer Research·Kok Poh LohYi Zhun Zhu
Jul 31, 2007·Environmental Research·Brian D CurwinMichael C Alavanja
Dec 25, 2007·Toxicology·Marilyn D SaulsburyDorothy Round
Jan 4, 2008·Journal of Cellular Biochemistry·Andy T Y LauJen-Fu Chiu
Jan 29, 2008·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Fu YuDecheng Bai
Mar 21, 2008·Ecotoxicology and Environmental Safety·A P K JantunenJ V K Kukkonen
Sep 16, 2008·PloS One·Howard Chia-Hao ChangSpyros Artavanis-Tsakonas
Jan 1, 2003·Toxicology Mechanisms and Methods·Aamir NazirD Kar Chowdhuri

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Citations

Sep 19, 2012·Toxicological Sciences : an Official Journal of the Society of Toxicology·Caridad López-GraneroFernando Sánchez-Santed
Oct 8, 2011·Disease Models & Mechanisms·Ethan Bier, Annabel Guichard
Feb 10, 2012·Archives of Environmental & Occupational Health·Rolando Payán-RenteríaRuth De Celis
Apr 25, 2014·Pesticide Biochemistry and Physiology·Dharmender Sharma, Gurinder Kaur Sangha
Jan 30, 2016·Pesticide Biochemistry and Physiology·Abhilasha ChaurasiaU S Gupta
Nov 1, 2015·The Journal of Steroid Biochemistry and Molecular Biology·Clara VenturaMariel Núñez
May 9, 2016·Pesticide Biochemistry and Physiology·Isaac A AdedaraJoao B T Rocha
Apr 3, 2016·Mutation Research. Reviews in Mutation Research·Maria AugustyniakMarta Dziewięcka
Feb 26, 2016·Journal of Toxicology and Environmental Health. Part a·Elena SturchioMauro Mecozzi
Apr 5, 2016·International Journal of Molecular Sciences·Antonio F Hernández, Pablo Menéndez
Jul 23, 2015·Toxicological Sciences : an Official Journal of the Society of Toxicology·Chengquan LuJing Shao
Aug 26, 2015·Journal of Trace Elements in Medicine and Biology : Organ of the Society for Minerals and Trace Elements (GMS)·Isaac A AdedaraJoao B T Rocha
Apr 30, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Ping MaXu Yang
Jan 27, 2015·Pesticide Biochemistry and Physiology·Dharmender SharmaKuldeep Singh Khera
Nov 1, 2015·Human & Experimental Toxicology·E M TanvirM I Khalil
Apr 17, 2019·Journal of Biochemical and Molecular Toxicology·Soheil FereidouniDevinder Kumar Dhawan
Oct 21, 2019·Environmental Science and Pollution Research International·Valeria Di NicaSara Villa
Mar 19, 2021·Saudi Journal of Biological Sciences·Shagufta TaqviSuriya Rehman
Dec 21, 2018·Ecotoxicology and Environmental Safety·Khaskhoussy KhawlaHachicha Mohamed

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