ChLpMab-23: Cancer-Specific Human-Mouse Chimeric Anti-Podoplanin Antibody Exhibits Antitumor Activity via Antibody-Dependent Cellular Cytotoxicity

Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
Mika K KanekoYukinari Kato

Abstract

Podoplanin is expressed in many cancers, including oral cancers and brain tumors. The interaction between podoplanin and its receptor C-type lectin-like receptor 2 (CLEC-2) has been reported to be involved in cancer metastasis and tumor malignancy. We previously established many monoclonal antibodies (mAbs) against human podoplanin using the cancer-specific mAb (CasMab) technology. LpMab-23 (IgG1, kappa), one of the mouse anti-podoplanin mAbs, was shown to be a CasMab. However, we have not shown the usefulness of LpMab-23 for antibody therapy against podoplanin-expressing cancers. In this study, we first determined the minimum epitope of LpMab-23 and revealed that Gly54-Leu64 peptide, especially Gly54, Thr55, Ser56, Glu57, Asp58, Arg59, Tyr60, and Leu64 of podoplanin, is a critical epitope of LpMab-23. We further produced human-mouse chimeric LpMab-23 (chLpMab-23) and investigated whether chLpMab-23 exerts antibody-dependent cellular cytotoxicity (ADCC) and antitumor activity. In flow cytometry, chLpMab-23 showed high sensitivity against a podoplanin-expressing glioblastoma cell line, LN319, and an oral cancer cell line, HSC-2. chLpMab-23 also showed ADCC activity against podoplanin-expressing CHO cells (CHO/podoplanin). In xen...Continue Reading

References

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Feb 28, 2015·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Hiroharu OkiYukinari Kato
Jun 20, 2015·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Mika K KanekoYukinari Kato
Jun 20, 2015·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Hiroharu OkiYukinari Kato
Oct 23, 2015·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Satoshi OgasawaraYukinari Kato
Mar 5, 2016·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Yukinari KatoMika Kato Kaneko
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Apr 8, 2017·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Shinji YamadaYukinari Kato

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Citations

Mar 27, 2018·Cancer Science·Harini KrishnanGary S Goldberg
Jun 5, 2018·Platelets·Katsue Suzuki-Inoue
Dec 21, 2018·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Yukinari KatoMika K Kaneko
Nov 21, 2018·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Shinji YamadaYukinari Kato
May 14, 2020·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Yukinari KatoMika K Kaneko
Apr 3, 2018·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Shinji YamadaYukinari Kato
Oct 27, 2018·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Shinji YamadaYukinari Kato
Sep 15, 2017·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Shunsuke ItaiYukinari Kato
Nov 19, 2020·Journal of Cancer Research and Clinical Oncology·Kelly L HamiltonGary S Goldberg
May 28, 2021·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Tomohiro TanakaMika K Kaneko
Aug 24, 2021·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Teizo AsanoYukinari Kato
Dec 28, 2021·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Teizo AsanoYukinari Kato

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Methods Mentioned

BETA
flow cytometry
xenograft

Software Mentioned

GraphPad
GraphPad PRISM

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