CHO expressed recombinant human lactoferrin as an adjuvant for BCG

International Journal of Immunopathology and Pharmacology
Shen-An HwangJeffrey K Actor

Abstract

Lactoferrin (LF), an iron binding protein with immune modulatory activities, has adjuvant activity to enhance vaccine efficacy. Tuberculosis (TB) is a pulmonary disease caused by the pathogen Mycobacterium tuberculosis (MTB). Progressive TB disease is clinically defined by damaging pulmonary pathology, a result of inflammation due to immune reactivity. The current vaccine for TB, an attenuated strain of Mycobacterium bovis, Bacillus Calmette Guerin (BCG), has only limited efficacy to prevent adult pulmonary TB. This study examines a Chinese hamster ovary (CHO) expressed recombinant human LF (rHLF) to boost efficacy of the BCG vaccine and delay early pathology post infectious challenge. C57BL/6 mice were immunized with BCG, or BCG admixed with either rHLF or bovine LF (bLF; internal control), or remained unvaccinated. Mice were then aerosol challenged with Erdman MTB. All vaccinated mice demonstrated decreased organ bacterial load up to 19 weeks post infection compared with non-vaccinated controls. Furthermore, mice receiving bLF or rHLF supplemented BCG vaccines showed a modest decrease in lung pathology developed over time, compared to the BCG vaccine alone. While mice vaccinated with BCG/rHLF demonstrated increased general lu...Continue Reading

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Citations

Mar 13, 2016·The American Journal of Pathology·Elizabeth DonnachieShen-An Hwang
Mar 4, 2017·International Journal of Molecular Sciences·Maria Elisa Drago-SerranoMireya de la Garza
Sep 22, 2017·Critical Reviews in Food Science and Nutrition·Bo WangBenu Adhikari
Nov 11, 2016·BMC Veterinary Research·Nada ZemankovaMartin Faldyna
Nov 23, 2017·Frontiers in Immunology·Javier Arranz-TrullénEster Boix
Mar 26, 2021·International Immunopharmacology·Michał ZimeckiMarian L Kruzel
May 1, 2021·Pharmaceuticals·Gabriel S OliveiraCátia Teixeira

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Datasets Mentioned

BETA
GM-CSF

Methods Mentioned

BETA
glycosylation
ELISA

Software Mentioned

Image J

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