Choanoflagellate transfection illuminates their cell biology and the ancestry of animal septins

Molecular Biology of the Cell
David S BoothNicole King

Abstract

As the closest living relatives of animals, choanoflagellates offer unique insights into animal origins and core mechanisms underlying animal cell biology. However, unlike traditional model organisms, such as yeast, flies and worms, choanoflagellates have been refractory to DNA delivery methods for expressing foreign genes. Here we report the establishment of a robust method for expressing transgenes in the choanoflagellate Salpingoeca rosetta, overcoming barriers that have previously hampered DNA delivery and expression. To demonstrate how this method accelerates the study of S. rosetta cell biology, we engineered a panel of fluorescent protein markers that illuminate key features of choanoflagellate cells. We then investigated the localization of choanoflagellate septins, a family of GTP-binding cytoskeletal proteins that are hypothesized to regulate multicellular rosette development in S. rosetta. Fluorescently tagged septins localized to the basal pole of S. rosetta single cells and rosettes in a pattern resembling septin localization in animal epithelia. The establishment of transfection in S. rosetta and its application to the study of septins represent critical advances in the growth of S. rosetta as an experimental mode...Continue Reading

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Citations

May 18, 2019·American Society of Clinical Oncology Educational Book·Rawad Elias, Oreofe Odejide
Sep 21, 2019·Development·Maria Sachkova, Pawel Burkhardt
May 16, 2019·Molecular Biology of the Cell·Jackie L Collier, Joshua S Rest
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Dec 5, 2020·Development·Iñaki Ruiz-Trillo, Alex de Mendoza
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May 20, 2021·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Jonas Muhr, Daniel W Hagey
May 28, 2021·ELife·Ashley L ArthurMargaret A Titus

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Methods Mentioned

BETA
transfection
fluorescence
flow cytometry
superresolution microscopy
flow
fluorescence-activated cell sorting
transgenics
transgenic
transfections
fluorescence-activated cell sorter

Software Mentioned

rosetta
Emboss

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