ChoG is the main inducible extracellular cholesterol oxidase of Rhodococcus sp. strain CECT3014

Microbiological Research
L Fernández de las HerasO Drzyzga

Abstract

Cholesterol catabolism has been reported in different bacteria and particularly in several Rhodococcus species, but the genetic of this complex pathway is not yet very well defined. In this work we report the isolation and sequencing of a 9.8 kb DNA fragment of Rhodococcus sp. strain CECT3014, a bacterial strain that we here identify as a Rhodococcus erythropolis strain. In this DNA fragment we found several ORF that are probably involved in steroid catabolism, and choG, a gene encoding a putative cholesterol oxidase whose functional characterization we here report. ChoG protein is a class II cholesterol oxidase with all the structural features of the enzymes of this group. The disruption of the choG gene does not alter the ability of strain CECT3014 cells to grow on cholesterol, but it abolishes the production of extracellular cholesterol oxidase. This later effect is reverted when the mutant cells are transformed with a plasmid expressing choG. We conclude that choG is the gene responsible for the inducible extracellular cholesterol oxidase activity of strain CECT3014. This activity distributes between the cellular membrane and the culture supernatant in a way that suggests it is produced by the same ChoG protein that occurs ...Continue Reading

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Citations

May 9, 2012·Applied Microbiology and Biotechnology·Marina V Donova, Olga V Egorova
Oct 16, 2013·The Journal of Steroid Biochemistry and Molecular Biology·Laura Fernández de Las HerasJuana María Navarro Llorens
Jan 31, 2013·Journal of Proteome Research·Ute HaußmannAnsgar Poetsch
May 31, 2011·Applied and Environmental Microbiology·O DrzyzgaJ Perera
Apr 22, 2014·Water Research·Christy M DykstraSpyros G Pavlostathis
Sep 30, 2014·Genome Génome / Conseil National De Recherches Canada·Wenjing LiLi Xu
Jul 10, 2012·The Journal of Steroid Biochemistry and Molecular Biology·Laura Fernández de las HerasJuana María Navarro Llorens
Mar 30, 2016·Bioresource Technology·Shubhrima Ghosh, S K Khare
Apr 1, 2012·Environmental Microbiology Reports·Iria UhíaJosé L García
Feb 9, 2012·Microbial Biotechnology·J L GarcíaB Galán
Jun 24, 2017·The Journal of Steroid Biochemistry and Molecular Biology·Govinda GuevaraJuana María Navarro Llorens
May 3, 2019·BMC Genomics·Govinda GuevaraJuana María Navarro-Llorens
Mar 16, 2017·Microbial Cell Factories·Govinda GuevaraJuana María Navarro Llorens
Dec 5, 2018·International Journal of Genomics·Hongyan ZhaoHongliang Huo

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