Choice of cell-delivery route for skeletal myoblast transplantation for treating post-infarction chronic heart failure in rat.

PloS One
Satsuki FukushimaKen Suzuki

Abstract

Intramyocardial injection of skeletal myoblasts (SMB) has been shown to be a promising strategy for treating post-infarction chronic heart failure. However, insufficient therapeutic benefit and occurrence of ventricular arrhythmias are concerns. We hypothesised that the use of a retrograde intracoronary route for SMB-delivery might favourably alter the behaviour of the grafted SMB, consequently modulating the therapeutic effects and arrhythmogenicity. Three weeks after coronary artery ligation in female wild-type rats, 5x10(6) GFP-expressing SMB or PBS only (control) were injected via either the intramyocardial or retrograde intracoronary routes. Injection of SMB via either route similarly improved cardiac performance and physical activity, associated with reduced cardiomyocyte-hypertrophy and fibrosis. Grafted SMB via either route were only present in low numbers in the myocardium, analysed by real-time PCR for the Y-chromosome specific gene, Sry. Cardiomyogenic differentiation of grafted SMB was extremely rare. Continuous ECG monitoring by telemetry revealed that only intramyocardial injection of SMB produced spontaneous ventricular tachycardia up to 14 days, associated with local myocardial heterogeneity generated by cluster...Continue Reading

References

Jun 22, 1999·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S R CoppenN J Severs
Apr 5, 2001·Nature·D OrlicP Anversa
Feb 20, 2002·Journal of Molecular and Cellular Cardiology·Jochen Müller-EhmsenLarry Kedes
Feb 20, 2002·Journal of Molecular and Cellular Cardiology·Hans ReineckeCharles E Murry
Jun 12, 2003·Journal of the American College of Cardiology·Craig A ThompsonStephen N Oesterle
Jun 12, 2003·Annals of the New York Academy of Sciences·Donald Orlic
May 18, 2005·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Harald C OttSteffen Hering
Jun 25, 2005·Circulation Research·M Roselle AbrahamEduardo Marbán
Mar 29, 2006·Heart Rhythm : the Official Journal of the Heart Rhythm Society·Kristen FoutsKenneth R Laurita
Apr 4, 2006·Nature Medicine·Yoshinori MiyaharaHidezo Mori
Apr 20, 2006·Clinical and Experimental Pharmacology & Physiology·F C HowarthE Adeghate
Dec 14, 2006·Transplantation·Maria Grazia OrofinoFrancesco Cucca
Dec 21, 2006·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Leanne E FelkinPaul J R Barton
Jul 13, 2014·American Journal of Physiology. Heart and Circulatory Physiology·Hongmei PengNour-Eddine Rhaleb

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Citations

Nov 19, 2010·Regenerative Medicine·Shazia DurraniKhawaja Husnain Haider
Mar 8, 2013·Future Cardiology·Satsuki FukushimaKen Suzuki
Dec 18, 2013·Stem Cell Research & Therapy·Zheyong HuangJunbo Ge
Jan 16, 2013·Molecular Therapy : the Journal of the American Society of Gene Therapy·Naosumi SekiyaJohnny Huard
Jan 18, 2012·Current Opinion in Cardiology·Peter DonndorfGustav Steinhoff
Jun 19, 2010·Journal of Cardiovascular Translational Research·Manuel MazoFelipe Prósper
Jun 1, 2012·Journal of Cardiovascular Translational Research·Niall G Campbell, Ken Suzuki
Jan 12, 2013·Journal of Cardiovascular Translational Research·Julianne H SpencerPaul A Iaizzo
Oct 12, 2010·Cardiovascular Revascularization Medicine : Including Molecular Interventions·Adam J SaltzmanSergio Waxman
May 27, 2009·Journal of Molecular and Cellular Cardiology·Yasunori ShintaniKen Suzuki
Apr 22, 2014·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Liudmila ZakharovaMohamed A Gaballa
Apr 14, 2016·Fertility and Sterility·Mats HellströmMats Brännström
Dec 15, 2015·Frontiers in Cardiovascular Medicine·Theofilos M KolettisZenon S Kyriakides
Feb 3, 2018·Journal of Cardiovascular Translational Research·Wouter A GathierSteven A J Chamuleau
Nov 16, 2018·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·Michaela BrychtovaLucie Vistejnova
Feb 28, 2019·Journal of Cellular Physiology·Seyed Mostafa ParizadehSeyed Mahdi Hassanian
Nov 15, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sara PahlavanHossein Baharvand
May 16, 2012·Phytotherapy Research : PTR·Gökçe TopalB Sönmez Uydeş Doğan
May 28, 2014·Heart Failure Reviews·Takuya Narita, Ken Suzuki
Mar 29, 2020·Stem Cell Research & Therapy·Lee Chuen LiewBoon-Seng Soh
Nov 6, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Reza RikhtegarMehdi Yousefi
Mar 7, 2020·European Journal of Pharmacology·Ziwei LiuXiaohui Zhou

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Methods Mentioned

BETA
coronary artery bypass
immunoconfocal microscopy
confocal microscopy
PCR
enzymatic dissociation

Software Mentioned

DSI Dataquest
NIH image analysis
NIH

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