Choice of endothelial marker is crucial for assessment of bone marrow microvessel density in chronic lymphocytic leukemia

APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica
L Smolej, P Kasparová

Abstract

Angiogenesis is a potential prognostic factor in chronic lymphocytic leukemia (CLL). Elevated circulating levels of angiogenic factors in CLL have been repeatedly reported. Nevertheless, the issue of bone marrow neovascularization in CLL remains controversial, partly due to limited number of published studies, different methods of assessing microvessel density (MVD) and small patient cohorts. Moreover, there are very scarce data regarding the relationship of marrow angiogenesis to prognostic markers in CLL. Our objectives were: 1. To assess bone marrow MVD in CLL using two different monoclonal antibodies and a reproducible method of MVD quantification; 2. To examine the possible association of marrow MVD and clinical course, pattern of marrow infiltration, Rai stage, cytogenetic abnormalities detected by fluorescence in situ hybridization (FISH), and mutation status of immunoglobulin heavy chain variable region (IgVH). MVD was higher using CD34 vs vWF antibody (p<0.0001). However, no MVD differences were detected between CLL subgroups subdivided according to the above-mentioned prognostic factors. In conclusion, MVD assessment using anti-CD34 resulted in higher MVD counts than when using anti-vWF antibody. No association of MVD...Continue Reading

References

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Citations

Jul 21, 2010·Medical Oncology·Shuyan MengZhou Caicun
Feb 26, 2010·Applied Immunohistochemistry & Molecular Morphology : AIMM·Darko AnticVladan Cokic
May 26, 2010·Leukemia & Lymphoma·Anna Szmigielska-KapłonTadeusz Robak
Sep 17, 2009·Acta Medica (Hradec Králové)·Vladimíra VroblováJan Krejsek

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