Cholate-induced dimerization of detergent- or phospholipid-solubilized bovine cytochrome C oxidase

Biochemistry
Andrej Musatov, N C Robinson

Abstract

Bovine heart cytochrome c oxidase (CcO), solubilized by either nonionic detergents or phospholipids, completely dimerizes upon the addition of bile salts, e.g., sodium cholate, sodium deoxycholate, or CHAPS. Bile salt induced dimerization occurs whether dodecyl maltoside, decyl maltoside, or Triton X-100 is the primary solubilizing detergent or the enzyme is dispersed in phosphatidylcholine, phosphatidylethanolamine, or mixtures thereof. In each case, complete CcO dimerization can be verified by sedimentation velocity and sedimentation equilibrium after correction for bound detergent and/or phospholipid. The relative concentration of the bile salt is critical for production of homogeneous, dimeric CcO. For example, enzyme solubilized by 2 mM detergent requires an equal molar concentration of sodium cholate. Similarly, enzyme dispersed in 20 mM phospholipid requires 50 mM sodium cholate, concentrations that are commonly used to reconstitute CcO into small unilamellar vesicles. Bile salts do more than just stabilize dimeric CcO and prevent detergent-induced dissociation into monomers. They are able to completely reverse detergent-induced monomerization and cause completely monomeric CcO to reassociate. Dimeric CcO so generated is...Continue Reading

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Citations

Sep 2, 2008·Biochemistry·Ling QinShelagh Ferguson-Miller
Aug 12, 2009·Biochemistry·Erik Sedlák, Neal C Robinson
Oct 12, 2012·The Journal of Biological Chemistry·Ingrid DachChristine Ebel
Sep 11, 2009·Antioxidants & Redox Signaling·Giorgio Lenaz, Maria Luisa Genova
Jul 12, 2013·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Andrej Musatov
Dec 18, 2014·Biophysical Journal·Erik SedlákNeal C Robinson
Jan 26, 2007·Analytical Biochemistry·Marc Le MairePhilippe Champeil
Oct 4, 2005·Mitochondrion·Oleh Khalimonchuk, Gerhard Rödel
Jul 20, 2014·Biochimie·Andrej Musatov, Neal C Robinson
Apr 25, 2006·Biochimica Et Biophysica Acta·Paul A CobineDennis R Winge
Apr 20, 2010·Biochimica Et Biophysica Acta·Lukas Stiburek, Jiri Zeman
Jul 2, 2016·Proceedings of the National Academy of Sciences of the United States of America·Idlir LikoCarol V Robinson
Mar 3, 2018·International Journal of Molecular Sciences·Renuka KolliChris Carrie
Jun 25, 2002·The Journal of Biological Chemistry·Denis JossePatrick Masson
Sep 20, 2019·Proceedings of the National Academy of Sciences of the United States of America·Kyoko Shinzawa-ItohKazumasa Muramoto
Jun 14, 2002·The Journal of Biological Chemistry·Heather S CarrDennis R Winge
Mar 7, 2021·Cells·Rabia RamzanSebastian Vogt
Jul 3, 2021·Cells·Rabia RamzanSebastian Vogt

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